植物种子传带病原螺原体的研究

经过1990—1993年的调查研究,明确了一些感染螺原体的植物的种子是能传带病原螺原体的,如大麦、菜豆、普通豇豆、长豇豆、赤豆、樟树、桤木、紫荆、紫茉莉、紫薇、桑树、木芙蓉、木槿及海州常山等10多种植物。并明确了种果的不同部位其螺原体含量不同,同一部位不同采集时期其所含螺原体数量也有差异。关于螺原体的分布扩散情况均为先到达果皮然后最终到达种子的种仁。关于豆科植物的种子的检测,由于其本身的吸收作用较强,必需采取转管培养的方式。 利用紫外吸收测定液体培养中的螺原体含量,是一种简便有效的方法,值得推广应用于植物及其种子中螺原体繁殖速度及数量比较上的检测等等。

Studies on Seed - borne Spiroplasma of Plants

After an investigation from 1990-1993 , plants such as Hordeum vulgare, Phaseolus vulgarise P- angularis, Vigna sinensis, V. Sesquipedalis, Cinnamomum camphora, Alnus cremastogyne,Cercis chinensis, Mirabilis jalapa, Lagerstroemia indica, Morus alba. Hibiscus niutabilis, H. syriactis, and Clerodendron tricholomum that infected Spiroplasma diseases, all were seed -borne host plants, We also found that the Spiroplsma contents of the seeds were differently according to their location on the seeds, and the same seeds position but collecting at various time had various contents. The pathogenic tran - ition of the Spiroplsma was firstly attached to the pericarp, at last it penetrated to the cotyledon. On the seed exemination of Legume crops, in view of its strong respiration, the transition test - tube culture are recommended under colour comparison. Using the Ultra - violet absorption method to exemining the Spiroplasma concentration in the liquid medium is a simple and useful method, that worth extendible to the comparison of reproductive speed and quantity of the pathogens in the plants and its seeds.