大豆单细胞培养和原生质体游离的细胞形态观察

本文将形状不同的大豆悬浮培养细胞分成三种类型;园形和椭园形(A类)稍长形(B类)和长形细胞(C类)。A类细胞分生能力强,经多次分裂形成多细胞团;C类细胞多数为老化细胞,能分裂但不能形成细胞团;B类细胞在悬浮细胞中含量很少,大部分能分裂形成细胞团。在用悬浮培养细胞游离原生质体的过程中,A类细胞几乎全能游离出原生质体,B类细胞大部分能游离出原生质体,C类细胞几乎不能游离出原生质体。因此,在游离悬浮培养细胞时,在原生质体中含有少量未去壁的C类细胞和极少数B类细胞可不必用其它的方法分离出去,因为C类细胞不能形成细胞团,那么经培养形成的愈伤组织和分化再生的完整植株可能几乎都是由原生质体产生的。

MORPHOCYTOLOGICAL OBSERVATION OF MONOCELL CULTURE AND PROTOPLAST ISOLATION IN SOYBEAN

We classify different suspension culture cells from soybean (Glycine max L.) in shape into three types: spherical and elliptic cells (A), slightly long cells (B) , long cells (C). The meristic ability of A-cells is strong, and A-cells can form into cell clusters through swecessive division. C-cells can divide, but can not form into long-cell clusters. C-cells are ageing cells. The case of B-cells division is situated between A and B. Protoplasts can fully be isolated from A-cells, and can not be-isolated from C-cells,in the process of protoplast isolation from suspension culture cell. Therefore, C-cells need not to be removed from prot oplasts using suspension culture cells as isolation material, the calli in culture fruid are from protoplasts.