| 外源DNA导入水稻的初步研究 |
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| 引用本文： | 张福泉,万文举,董延瑜,洪亚辉. 外源DNA导入水稻的初步研究[J]. 湖南农业大学学报(自然科学版), 1992, 0(2) |
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| 作者姓名： | 张福泉 万文举 董延瑜 洪亚辉 |
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| 作者单位： | 湖南农学院农学系，湖南农学院农学系，湖南农学院农学系，湖南农学院农学系 长沙，410128，长沙，410128，长沙，410128，长沙，410128 |
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| 摘 要： | 设计了不同药剂和接种方法处理的胚培法将玉米DNA导入水稻的试验,对导入后代进行单株产量的分析结果表明:a.水稻采用一般胚培法(MS+DNA+胚)导入玉米DNA,未能产生主要经济性状(单侏产量)的有利变异;b.胚经不同药剂(丙酮、氯化钙和十二烷基硫酸钠)处理后,能促进外源DNA的导入;c.采用4%丙酮浸胚12h后。再接种到培养基(MS+DNA)上的方法,导入效果较好。
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| 关 键 词： | 水稻 外源 脱氧核糖核酸 胚培养 经济性状 |
A STUDY ON THE INTRODUCTION OF FOREIGN DNA INTO RICE |
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| Zhang Fuquan,Wan Wenju,Dong Yanyu,Hong Yahui,. A STUDY ON THE INTRODUCTION OF FOREIGN DNA INTO RICE[J]. Journal of Hunan Agricultural University, 1992, 0(2) |
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| Authors: | Zhang Fuquan Wan Wenju Dong Yanyu Hong Yahui |
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| Abstract: | A method of embryo culture with different chemicals and inocula-tion ways were used to introduce foreign DNA. The result is as follews based on theanalysis of single plant yield.a) General method(MS+DNA+embryo)to introducemaize DNA to rice doesn't produce favorable variation of main economic char-acter(single plant yield).b)The treatments of different chemicals to embryo helpintroduce foreign DNA.c)A more effective method is to soak embryo with Ace-tone (12h) and then inoculate to culture(MS+DNA). |
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| Keywords: | lowland rice exogenous decxyribonucleic embryo culture economic character |
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