Canine normal corneal epithelium bears a large population of CD45-positive cells |
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Authors: | Carvalho Andrea R R Naranjo Carolina Leiva Marta Fondevila Dolors Iborra Antoni Martinez Paz Peña Teresa |
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Affiliation: | aDepartament de Medicina i Cirurgia Animals, Universitat Autònoma de Barcelona, 08193 Bellaterra (Cerdanyola del Vallès), Barcelona, Spain;bInstitut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, 08193 Bellaterra (Cerdanyola del Vallès), Barcelona, Spain |
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Abstract: | This investigation sought to identify the presence of immune cells in normal canine corneal epithelium. A whole-mount immunofluorescence study of normal canine epithelium using monoclonal antibodies against CD45, CD11c, CD1c and MHC class II was performed. CD45-positive cells were located in all epithelial layers throughout the cornea, occurring in greater numbers (51.98 ± 4.1/mm2) at the periphery and decreasing towards the central region (11.8 ± 3.1/mm2). CD11c-positive cells were also observed, but were fewer in number. The findings show that the normal canine cornea carries a significant number of cells of immune origin; these cells seem to be of an inactive phenotype as they do not express MHC class II. Further studies are needed to determine whether these cells can express co-stimulatory molecules and act as antigen presenting cells if stimulated. |
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Keywords: | Dog Cornea Epithelium CD45 Leucocyte |
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