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A stereological method for estimating the feto-maternal exchange surface area in the bovine placentome at 135 days gestation |
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| Authors: | Kannekens E M Murray R D Howard C V Currie J |
| Affiliation: | a Utrecht University Faculty of Veterinary Medicine, Department of Farm Animal Health, Ruminant Health Unit, The Netherlands b University of Liverpool Departments of Veterinary Science, Veterinary Teaching Hospital, Leahurst, Neston, UK c School of Biomedical Sciences, University of Ulster at Coleraine, Co. Londonderry BT52 1SA, UK d Human Anatomy and Cell Biology, Ashton Street, Liverpool, UK |
| Abstract: | The feto-maternal relationships within the bovine placentome at 135 days gestation were investigated using unbiased stereology. This provides an estimate of the feto-maternal interface without physically separating these. The functional surface area, the volume of the fetal and maternal components, volumetric tissue shrinkage due to processing, and the feto-maternal surface amplification factor were estimated and quantified in standard units. In this healthy bovine pregnancy there were 105 placentomes with a total volume of 900 ml.The volumetric tissue shrinkage was 24% and the maternal: fetal tissue ratio overall was 0.92. The total feto-maternal exchange surface area was 18.5 m2 with a surface amplification factor of 108.1. Analysis of the coefficient of error from counting different numbers of points within the reference area suggested that 50% and 75% fewer points could be counted to achieve the result obtained by counting approximately 6000 points used in this study. |
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