| 蒙古扁桃的组织培养与快速繁殖技术研究 |
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| 引用本文： | 吴建华,王立英,李健.蒙古扁桃的组织培养与快速繁殖技术研究[J].安徽农业科学,2010,38(4):1733-1734. |
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| 作者姓名： | 吴建华 王立英 李健 |
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| 作者单位： | 种苗生物工程国家重点实验室,宁夏银川,750004;宁夏林业研究所,有限公司,宁夏银川,750004;种苗生物工程国家重点实验室,宁夏银川,750004;宁夏林业研究所,有限公司,宁夏银川,750004 |
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| 摘 要： | 目的]对蒙古扁桃（Prunus mongolica Maxim.）的组织培养与快速繁殖技术进行研究。方法]以蒙古扁桃幼嫩茎段为外植体进行组织培养试验。结果]筛选出适合蒙古扁桃外植体的诱导培养基为MS＋6-BA0.80mg/L＋IAA1.20mg/L＋30g/L蔗糖＋5g/L琼脂粉,pH值为6.0～6.2;愈伤组织诱导培养基为MS＋6-BA1.00mg/L＋NAA0.10mg/L＋30g/L蔗糖＋5g/L琼脂粉,pH值为6.0～6.2;继代芽分化培养基为MS＋6-BA0.80mg/L＋IAA1.50mg/L＋NAA0.05mg/L＋30g/L蔗糖＋5g/L琼脂粉,pH值为6.0～6.2;生根培养基为1/2MS＋IBA0.50mg/L＋根皮苷5.00mg/L＋15g/L蔗糖＋5g/L琼脂粉,pH值为6.0～6.2;以草炭：蛭石：珍珠岩=2：1：1为移栽基质效果最好。结论]为蒙古扁桃品种改良及工厂化繁育提供参考。
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| 关 键 词： | 蒙古扁桃 组织培养 快速繁殖 |
Study on Tissue Culture and Rapid Propagation of Prunus mongolica Maxim. |
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| WU Jian-hua et al.Study on Tissue Culture and Rapid Propagation of Prunus mongolica Maxim.[J].Journal of Anhui Agricultural Sciences,2010,38(4):1733-1734. |
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| Authors: | WU Jian-hua |
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| Institution: | State Key Laboratory of Seedling Bioengineering of Ningxia;Yinchuan;Ningxia 750004 |
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| Abstract: | Objective]The research aimed to study the tissue culture and rapid propagation of Prunus mongolica Maxim.Method]Young stems were used as explants to study tissue culture of Prunus mongolica Maxim.Result]The results showed that the best explant induction medium was MS + 6-BA 0.80 mg/L + IAA1.20 mg/L + 30 g/L Sugar + 5 g/L agar powder(pH value 6.0-6.2),the callus inducton medium was MS + 6-BA 1.00 mg/L + NAA 0.10 mg/L + 30 g/L Sugar + 5 g/L agar powder(pH value 6.0-6.2),the shoot multiplication medium was ... |
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| Keywords: | Prunus mongolica Maxim Tissue culture Rapid propagation |
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