Comparison of complement fixation test,competitive ELISA and LppQ ELISA with post-mortem findings in the diagnosis of contagious bovine pleuropneumonia (CBPP) |
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Authors: | Muuka Geofrey Hang’ombe Bernard Mudenda Nalubamba King Shimumbo Kabilika Swithine Mwambazi Lucas Muma John Bwalya |
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Institution: | (1) Central Veterinary Research Institute, Ministry of Livestock and Fisheries Development, P.O. Box 33980, Lusaka, Zambia;(2) Department of Paraclinical Studies, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka, Zambia;(3) Department of Clinical Studies, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka, Zambia;(4) Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka, Zambia; |
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Abstract: | The complement fixation test (CFT), the c-ELISA and an indirect LppQ ELISA were compared to post-mortem (PM) inspection for
the diagnosis of contagious bovine pleuropneumonia (CBPP). Sera from 797 cattle in the CBPP affected area of Kazungula, Zambia
and 202 sera from Lusaka, Zambia, a CBPP-free area were used. The clinical history of CBPP was recorded and all the cattle
from Kazungula were slaughtered and PM inspections conducted. The prevalence of CBPP in Kazungula was 67.5% (95%CI 67.2%,
70.8%), 52.6% (95%CI 49.2%, 56.2%), 59.0% (95%CI 55.5%, 62.4%) and 44.4% (95%CI 41.0%, 47.9%) using PM inspection, CFT, c-ELISA
and LppQ ELISA, respectively. Three of the 202 negative control animals tested positive on the c-ELISA although they were
from a known CBPP negative zone. In this study, the c-ELISA was more sensitive in detecting cattle with lesions in the chronic
stage than any other test whilst the CFT detected more during the onset stage. No single serological test could detect all
stages of CBPP infection, therefore the use of more than one test is advised. |
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