Calf production from vitrified bovine sexed embryos following in‐straw dilution |
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Authors: | Kiyoshi AKIYAMA Jun KOBAYASHI Yoshimasa SATO Ryuichi SATA Megumi OHASHI Emi SASAKI Yorimasa ODA Yoshio OGAWA Shuji UEDA Hisashi NABENISHI Satoko MATOBA |
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Institution: | 1. Kanagawa Prefectural Livestock Industry Technical Center, Ebina, Kanagawa,;2. Fukushima Agricultural Technology Centre, Fukushima, Fukushima,;3. Niigata Agricultural Reserch Institute, Sanjo, Niigata,;4. Tochigi Prefectural Dairy Farming Experiment Station, Shiobara, Tochigi,;5. Ishikawa Livestock Reseach Center, Houdatsushimizu, Ishikawa,;6. Shimane Prefectural Livestock Technical Center, Izumo, Shimane,;7. Okayama Prefectural Center of Animal Husbandry and Research, Misaki, Okayama,;8. Yamaguchi Prefectural Livestock Research Institute, Mine, Yamaguchi,;9. Hukuoka Agricultural Research Center, Chikushino, Fukuoka,;10. Miyazaki Livestock Research Institute, Takaharu, Miyazaki and;11. National Livestock Breeding Center, Nishigo, Fukushima, Japan |
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Abstract: | The objective of this study was to develop an in‐straw dilution method suitable for direct transfer of vitrified bovine sexed embryos. Embryo sexing was performed by molecular diagnosis. Several sexed and vitrified‐warmed embryos were transferred after evaluation of morphologically embryonic survival at warming and in‐straw dilution (Evaluation group). The other embryos were immediately directly transferred to recipients without first being expelled from the straws after in‐straw dilution (Non‐evaluation group). The pregnancy rates of vitrified sexed embryos were 38.7% and 34.8% in the Evaluation group and Non‐evaluation group, respectively, which were not significantly different. The viability of lower quality embryos before vitrification tended to be lower (P = 0.087) than that of the higher quality embryos regardless of evaluating embryos after warming and in‐straw dilution. The abortion rates were similar, and there was no difference between the two groups (13.9% and 12.5%, respectively). These results demonstrate that vitrified bovine sexed embryos can be vitrified and diluted by the in‐straw method and that the vitrified and warmed sexed embryos can develop to term. |
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Keywords: | bovine direct transfer in‐straw dilution sexed embryo vitrification |
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