仔猪产气荚膜梭菌肠毒血症病原诊断及疫苗研究V.疫苗的制备、检验和田间试验

将试验确定的产毒力强的产气荚膜梭菌A型、C型菌种,选用复合培养基,经培养、灭活脱毒、浓缩和冷冻干燥,制备多批肠毒血症A型、C型和AC型二价干粉灭活疫苗.采用免疫攻毒或中和试验方法对研制的疫苗进行效力检验,疫苗100%合格,达到<规程>规定的标准.在13个省进行大规模田间试验的结果表明,所研制的疫苗性能良好,安全有效.母猪接种疫苗后无一例不良反应发生,并且仔猪生后健康,能够有效地预防和控制该病的发生和流行.

Pathogenic diagnosis of enterotoxeamia caused by Clostridium perfringens in piglets and development of vaccines Ⅴ.Preparation,assay and field application of the vaccines

Several batches of killed monovalent and bivalent vaccines against piglet's enterotoxeamia caused by Clostridium perfrigens were prepared with selected strains of Clostridium perfrigens type A and type C which have stronger toxigenic capacities by the technological precess of cultivation,sterilization and detoxification, concentration and lyophilization using a compound medium. Potence of vaccines were tested by toxin challenge or toxin-antitoxin neutralization tests. The results showed that vaccines were safe and efficient. Pilot uses of vaccines in fields (demonstrated) that the vaccines were good in safety and efficiency and could prevent and control piglet's Clostridium perfringens enterotoxeamia efficaciously.