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金银花中绿原酸提取工艺的正交优化法研究
引用本文:李宁,李楚.金银花中绿原酸提取工艺的正交优化法研究[J].山西农业科学,2010,38(3):14-15,22.
作者姓名:李宁  李楚
作者单位:1. 山西医科大学药学院,山西,太原,030001
2. 山西省中医药研究所,山西,太原,030001
摘    要:运用正交设计,研究了乙醇浓度、乙醇量、回流次数和回流时间4个因素对金银花中绿原酸提取工艺的影响,并用紫外吸收法对其绿原酸的含量进行了测定。结果表明,75%乙醇,用量60 mL,回流2次,每次1 h为最佳提取条件。

关 键 词:金银花  绿原酸  正交设计  紫外分光光度法

The Orthogonal Experimental Analyze on the Extracting Technology of Chlorogenic Acid from Honeysuckle
LI Ning,LI Chu.The Orthogonal Experimental Analyze on the Extracting Technology of Chlorogenic Acid from Honeysuckle[J].Journal of Shanxi Agricultural Sciences,2010,38(3):14-15,22.
Authors:LI Ning  LI Chu
Institution:LI Ning1,LI Chu2(1.College of Pharmacy,Shanxi Medical University,Taiyuan 030001,China,2.Research Laboratory of Traditional Chinese Medicine in Shanxi Province,China)
Abstract:The four factors are the concent ration of ethanol,volume of solvent,extraction times and extraction hour.The effect of four factors on the concent ration of chlorogenic acid in flos lonicerae is examined by orthogonal design and ultraviolet absorption method.The result showed that 75% ethanol,60 mL,2 times,1 h each time is the best extraction method.
Keywords:Honeysuckle(Flos lonicerae)  Chlorogenie acid  Orthogonal design  Ultraviolet spectrop hotomet ric method
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