In vitro shoot regeneration ofSolanum tuberosum cultivars: interactions of medium composition and leaf, leaflet, and explant position |
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Authors: | J Hansen B Nielsen S V S Nielsen |
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Institution: | 1. Department of Ornamentals, Danish Institute of Agricultural Sciences, Kirstinebjergvej 10, DK-5792, ?rslev, Denmark 2. Biotechnology Group, Danish Institute of Agricultural Sciences, Lottenborgvej 2, DK-2800, Lyngby, Denmark
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Abstract: | Summary Shoot regeneration was investigated on explants from different leaves and leaflets of three potato cultivars Posmo, Folva
and Oleva. Explants were excised from glasshouse grown plants and grown for 6 days on callus induction medium with indole-3-acetic
acid or 2,4-dichlorophenoxyacetic acid. Explants were then transferred to auxin free shoot regeneration medium with gibberellic
acid and 6-benzyladenine or zeatin. By using the optimum combinations and concentrations of plant growth regulators and by
excision of explants from particular regions of proximal leaflets from newly unfolded leaves, shoot regeneration frequencies
of 97.0% were obtained for cv. Posmo and 32.1% for cv. Folva. Shoot regeneration frequency of cv. Oleva was very low and could
not be improved by the different treatments. |
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Keywords: | auxin cytokinin potato |
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