高粱体细胞克隆变异化学诱导方法研究

高粱组培育种仍以传统的常规培养技术为主，变异属于自然发生，变异频率不太高，因而制约了该方法应用的有效性。此研究旨在通过采用化学方法进行人工诱变以期达到提高诱变率的目的，采用的试验材料以高粱主要恢复系三尺三和晋粱5号为主，诱变剂以硫酸二乙酯为主。由于对生物体具有诱变效果的化学物质一般对人体具有很大毒性，所以探索安全无污染的处理方法则是非常必要的。通过几年的研究探索，达到了预期的目的，创立了有毒化学物质安全无污染诱变处理方法，同时明确了硫酸二乙酯诱变处理高粱愈伤组织的浓度一般为0.5%~1%。

Studies on Chemical Induction of Somaclonal Variations in Sorghum

Sorghum traditional tissue culture technology was still used in inducing somaclonal variations,which meant the somaclonal variations occurred naturally and the frequency of variations was not very high,thus restricting the application effectiveness.This study aimed to enhance frequency of variations through the artificial chemical induction of somaclonal variations.The test materials used in the study were R-line San Chi San and Jin liang No 5.The chemical mutagen was diethyl sulphate.Generally,the chemical mutagen was poisonous to organisms and therefore it was important to establish safty treatment procedures without pollution.Through years of research and exploration,safety and pollution-free Procedure for treating callus with toxic chemicals was established.The results showed that 0.5%-1% concentrations of DES was suitable to treat sorghum calli.