| 酸枣组培快繁的影响因素 |
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| 引用本文： | 代丽,王玖瑞,刘孟军,赵锦,周俊义,刘晓光,刘彩霞. 酸枣组培快繁的影响因素[J]. 河北农业大学学报, 2006, 29(3): 26-28,42 |
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| 作者姓名： | 代丽 王玖瑞 刘孟军 赵锦 周俊义 刘晓光 刘彩霞 |
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| 作者单位： | 河北农业大学,中国枣研究中心,河北,保定,071001;河北农业大学,林学院,河北,保定,071001;河北农业大学,生命学院,河北,保定,071001 |
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| 基金项目： | 国家专项基金 , 国家发改委资助项目 , 河北农业大学校科研和教改项目 |
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| 摘 要： | 试验研究了酸枣组培快繁体系优化过程中的影响因素,结果表明:休眠期水培新梢是酸枣快繁启动培养的适宜外植体,应采用70%C2H5OH+0.1%HgCl2浸泡6~8 min的方法进行表面消毒;增殖培养以20 d或30 d为一个继代周期为宜;暗培养10 d可以显著促进试管苗生根,而基部刻伤对试管苗生根率和生根条数无明显影响。
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| 关 键 词： | 酸枣(Ziziphus acidojujuba) 组培 快繁 影响因素 |
| 文章编号： | 1000-1573（2006）03-0026-03 |
| 收稿时间： | 2006-03-01 |
| 修稿时间： | 2006-03-01 |
Study on factors effecting micropropagation in wild jujube(Ziziphus acidojujuba) |
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| DAI Li,WANG Jiu-rui,LIU Meng-jun,ZHAO Jin,ZHOU Jun-yi,LIU Xiao-guang,LIU Cai-xia. Study on factors effecting micropropagation in wild jujube(Ziziphus acidojujuba)[J]. Journal of Agricultural University of Hebei, 2006, 29(3): 26-28,42 |
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| Authors: | DAI Li WANG Jiu-rui LIU Meng-jun ZHAO Jin ZHOU Jun-yi LIU Xiao-guang LIU Cai-xia |
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| Affiliation: | 1. Research Center of Chinese Jujube, Agricultural University of Hebei, Baoding 071001, China; 2. College of Forestry, Agricultural University of Hebei, Baoding 071001, China; 3. College of Life Science, Agricultural University of Hebei, Baoding 071001, China |
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| Abstract: | Factors influencing micropropagation were optimized for wild jujube(Ziziphus acidojujuba C.Y.Cheng et M.J.Liu).The new shoots from dormancy branch stem cultured in water were ideal explants during initiation culture.Explants could be rinsed with 70%C_2H_5OH for 30 sec and then mith 0.1%HgCl_2 for 6-8 min to establish sterilized system.Subculture period was 20 or 30 d during efficient proliferation.While the growth of roots was obviously accelerated by dark culture for 10 d,the rooting rate and amount of roots every shoot were not efficiently influenced by wound at shoot base. |
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| Keywords: | wild jujube tissue culture micropropagation factors |
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