| 丙肝病毒融合抗原基因导入烟草叶绿体及转化株同质化的研究 |
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| 引用本文： | 山松,张中林,吴祥甫,沈桂芳.丙肝病毒融合抗原基因导入烟草叶绿体及转化株同质化的研究[J].作物学报,2000,26(2):143-147. |
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| 作者姓名： | 山松 张中林 吴祥甫 沈桂芳 |
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| 作者单位： | 中国农业科学院生物技术研究中心， 北京， 100081 |
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| 摘 要： | 植物生物反应器的研究已成为当前基因工程领域的一个新生长点。 本实验用PCR方法， 从丙型肝炎病毒cDNA文库中克隆了非结构NS3区基因片段及核心抗原C区基因片段， 并在两段基因间加入连接肽SPGS的密码子序列， 构建成融合抗原基因NS3-C。 将该融合基因转入烟草叶绿体转化及表达载体中， 通过基因枪转化法得到转基因植株。 对N
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| 关 键 词： | 丙型肝炎病毒 融合抗原基因 烟草 叶绿体表达体系 同质化 |
| 收稿时间： | 1998-11-30 |
| 修稿时间： | 1999-06-18 |
A Chimeric Antigen Gene of Hepatitis C Virus was Introduced into Chloroplast of Tobacco & the Study of Transgenic Plant Homogenization |
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| SHAN Song,ZHANG Zhong-Lin,WU Xiang-Fu,SHEN Gui-Fang.A Chimeric Antigen Gene of Hepatitis C Virus was Introduced into Chloroplast of Tobacco & the Study of Transgenic Plant Homogenization[J].Acta Agronomica Sinica,2000,26(2):143-147. |
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| Authors: | SHAN Song ZHANG Zhong-Lin WU Xiang-Fu SHEN Gui-Fang |
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| Institution: | Biotechnology Research Center, Chinese Academy of Agricultural Sciences, Beijing, 100081 |
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| Abstract: | Spectinomycin-resistant calli and shoots were obtained from tobacco leaves bombarded with pSS3 DNA which contains the NS3-C chimeric antigen gene of hepatitis C virus. Further molecular screening on the transformed tobacco plants such as Southern blot and PCR amplifying were performed, which indicat |
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| Keywords: | Hepatitis C virus Chimeric antigen gene Chloroplast expression system Biolistic transformation Homogenization |
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