| 辣椒抗疫病研究中的RAPD反应体系优化 |
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| 引用本文： | 胡勇胜,毛胜利,刘明月,张宝玺.辣椒抗疫病研究中的RAPD反应体系优化[J].辣椒杂志,2007,5(1):5-8. |
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| 作者姓名： | 胡勇胜 毛胜利 刘明月 张宝玺 |
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| 作者单位： | 1. 湖南农业大学园艺园林学院,长沙,410128
2. 中国农业科学院蔬菜花卉研究所,北京,100081 |
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| 基金项目： | 国家“863”计划项目 |
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| 摘 要： | 以高抗疫病的perenial、高感疫病的83-60,及perenial×83-60的F2代辣椒苗为材料,采用CTAB法提取辣椒基因组DNA,研究RAPD-PCR反应的相关影响因子,建立并优化了一组适合辣椒抗疫病研究的RAPD-PCR反应体系和程序。
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| 关 键 词： | 辣椒 RAPD 疫病 |
| 收稿时间： | 2007-02-07 |
| 修稿时间： | 2007年2月7日 |
Optimization of RAPD System for Studying the Resistance to Phytophthora Blight in Hot Pepper |
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| Hu Yongsheng,Mao Shengli,Liu Mingyue,Zhang Baoxi.Optimization of RAPD System for Studying the Resistance to Phytophthora Blight in Hot Pepper[J].Journal of China Capsicum,2007,5(1):5-8. |
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| Authors: | Hu Yongsheng Mao Shengli Liu Mingyue Zhang Baoxi |
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| Abstract: | Perenial which is highly resistant to phytophthora blight, 83-60 which is highly susceptible to phytophthora blight, and their F2 progeny were used as experimental materials. The genomic DNA was isolated with CTAB method. The optimal RAPD system was set up by analyzing the key factors involved in the reaction system to study the resistance to phytophthora blight in pepper. |
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| Keywords: | hot pepper RAPD (random amplified polymorphic DNA) phytophthora blight |
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