CO2体积浓度对酒精废水培养微藻及其净化效果的影响

从4种常见微藻中筛选出能够充分利用木薯酒精废水及CO2废气生长的种类,并探究CO2体积浓度对其生长及净化废水中总氮(TN)、总磷(TP)、化学需氧量(CODCr)能力的影响。通过微藻在废水中生长的干重变化及叶绿素a(Chl-a)含量判断其生长情况,筛选出最适合的微藻。通过单因子试验,研究不同CO2体积浓度对筛选微藻的干重及叶绿素a(Chl-a)含量,以及CO2固定速率的影响,来寻找最适合生长的质量浓度。通过测定废水中TN、TP、CODCr的变化来评估净化作用。筛选试验结果表明,钝顶螺旋藻(Spirulina platensis)能适应酒精废水和CO2废气中的生长环境,在未经稀释的酒精废水中,其干重和Chl-a均显著高于波吉卵囊藻、普通小球藻和四尾栅藻(P<0.05)。单因子试验结果显示,当通入体积浓度2.5%的CO2,在酒精废水中培养的钝顶螺旋藻的干质量为(1.62±0.05)g/L、Chl-a为(12.1±0.7)mg/L,均高于其他试验组(P<0.05)。同时其对酒精废水中TN、TP、CODCr的去除率显著最高,分别为(50.5±2.1)%、(73.2±2.0)%及(24.9±0.6)%。钝顶螺旋藻能够适应酒精废水的生长环境,能吸收利用CO2废气,并有不错的净化效果。最适合其生长的CO2体积浓度是2.5%。

Effect of CO2 volume concentration on Microalgae cultured in alcohol wastewater and its purification

The species that can make full use of cassava alcohol wastewater and CO2 exhaust gas to grow were screened from four common species of common Microalgae,and the effects of CO2 concentration on their growth and ability to remove total nitrogen(TN),total phosphorus(TP)and chemical oxygen demand(CODCr)in wastewater were investigated.The growth of Microalgae was judged by the change of dry weight and chlorophyll a(Chl-a)content in wastewater,and the most suitable Microalgae was selected.Through single factor test,the effects of different CO2 volume concentrations on the dry weight,Chl-a content and fixed rate of CO2 of selected Microalgae were studied to find the most suitable concentration for growth.The purification effect was evaluated by measuring the changes of TN,TP and CODCr in wastewater.Screening test showed that Spirulina platensis could adapt to the growth environment of alcohol wastewater and CO2 exhaust gas.In 100%alcohol wastewater,the dry weight and Chl-a content of S.platensis were significantly higher than those of Oocystis borgei,Chlorella vulgaris and Scenedesmus quadricauda(P<0.05).The single factor test showed that when 2.5%CO2 was added,the dry weight of S.platensis cultured in alcohol wastewater was(1.62±0.05)g/L,and the Chl-a content was(12.1±0.7)mg/L,both significantly higher than those in other test groups(P<0.05).Meanwhile,the removal rates of TN,TP and CODCr in alcohol wastewater were(50.5±2.1)%,(73.2±2.0)%and(24.9±0.6)%respectively.S.platensis could adapt to the growth environment of alcohol wastewater and absorb CO2 exhaust gas,with good purification effect.The most suitable CO2 volume concentration for its growth is 2.5%.