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The Finding and Phylogenetic Evolution Analysis of Bovine Piroplasms in the Rasn Area of North Korea
Authors:Li-jun JIA  Shou-fa ZHANG  Shi-nuo CAO  Nian-chao QIAN  Long-zheng YU  Xue-nan XUAN
Institution:1. Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Yanbian University, Yanji 133002, P.R. China;2. National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan
Abstract:The objective of this study was to investigate the epidemiology of bovine Piroplasms infections in the Ras
></figure>n area of North Korea. The survey was carried out by light microscopic examination of Giemsa-stained blood smears, PCR, and phylogenetic evolution analysis of 128 blood samples collected from the Ras<figure class=></figure>n area. The results showed that the infection rates of the small and large parasites were about 2.5 and 1.5% on average, respectively, in all <em>Theileria sergenti</em> and <em>Babesia ovata</em> positive blood smears by microscopic examination of blood smears. The detection rate of <em>T. sergenti</em> Giemsa-stained smears was 43.75%, while that with PCR was 67.97%. The detection rate of <em>B. ovata</em> Giemsa-stained smears was 49.21%, while that with PCR was 71.88%. The sequence and phylogenetic analysis of DNA showed 98.84% homology between the 18S rRNA gene sequences of <em>T. sergenti</em> isolates from North Korean and that of Yanbian state from China, indicating the closest genetic relationship between both of them. Moreover, 100% homology was shown between the 18S rRNA gene sequence of <em>B. ovata</em> isolates from North Korea and the published sequence AY081192 of GenBank, indicating the closest genetic relationship between both of them. This survey confirmed that Ras<figure class=></figure>n is the endemic area of <em>T. sergenti</em> and <em>B. ovata</em> in North Korea.</td>
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Keywords:phylogenetic trees
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