| 湖南桂阳马尾松种子园遗传多样性的ISSR分析 |
| |
| 引用本文： | 杨玉洁,张冬林,杨模华,李志辉,唐效蓉,丁贵杰,谭晓风.湖南桂阳马尾松种子园遗传多样性的ISSR分析[J].中南林业科技大学学报,2010,30(3). |
| |
| 作者姓名： | 杨玉洁 张冬林 杨模华 李志辉 唐效蓉 丁贵杰 谭晓风 |
| |
| 作者单位： | 杨玉洁,杨模华,李志辉,YANG Yu-jie,YANG Mo-hua,LI Zhi-hui(中南林业科技大学林学院,湖南,长沙,410004);张冬林,ZHANG Dong-lin(中南林业科技大学林学院,湖南,长沙,410004;缅因大学,美国,04469);唐效蓉,TANG Xiao-rong(湖南省林业科学院,湖南,长沙,410004);丁贵杰,DINg Gui-jie(贵州大学林学院,贵州,贵阳,550025);谭晓风,TAN Xiao-feng(中南林业科技大学经济林育种与栽培国家林业局重点实验室,湖南,长沙,410004) |
| |
| 基金项目： | "十一五"国家科技支撑计划课题，教育部高等学校博士学科点专项科研基金，湖南省教育厅重点项目，湖南省教育厅优秀青年项目 |
| |
| 摘 要： | 本研究从100条ISSR引物中筛选出11条多态性引物,对湖南桂阳马尾松第二代种子园共87份样品进行遗传多样性分析,共扩增得到246个多态位点。采用PAUP4.0软件分析,获得两个主要群体(广西群体和城步群体),在城步群体内,湖南和贵州两省的家系被分开。再对两个主要群体采用POPGEN32软件分析,结果表明:在桂阳第二代种子园内总的多态位点百分率达98.80%,总的Shannon信息指数为0.583 7,Nei's基因多样性指数为0.400 3,2个群体的多态位点百分率分别为93.57%、98.80%,各群体间的基因分化系数为0.036 3,基因流为13.258 6。这表明桂阳第二代马尾松种子园的遗传多样性水平高,个体间的基因多样性变异大,该种子园所采用的建园方式是可取的。
|
| 关 键 词： | 森林培育学 马尾松 种子园 遗传多样性 |
Genetic diversity of Pinnus massoniana seed orchard of Guiyang using ISSR markers |
| |
| YANG Yu-jiea,ZHANG Dong-lina,YANG Mo-huaa,LI Zhi-huia,TANG Xiao-rong,DING Gui-jie,TAN Xiao-fengb.Genetic diversity of Pinnus massoniana seed orchard of Guiyang using ISSR markers[J].Journal of Central South Forestry University,2010,30(3). |
| |
| Authors: | YANG Yu-jiea ZHANG Dong-lina YANG Mo-huaa LI Zhi-huia TANG Xiao-rong DING Gui-jie TAN Xiao-fengb |
| |
| Institution: | YANG Yu-jie1a,ZHANG Dong-lin1a,2,YANG Mo-hua1a,LI Zhi-hui1a,TANG Xiao-rong3,DING Gui-jie4,TAN Xiao-feng1b(1.a.College of Forestry,b.The Key Laboratory of Non-timber Breeding & Cultivation of National Forestry Ministry,Central South University of Forestry , Technology,Changsha 410004 Hunan,China,2.University of Maine,Oromo 04469,USA,3.Hunan Forestry Academy 410004,4.College of Forestry,Guizhou University,Guiyang,550025 Guizhou,China) |
| |
| Abstract: | Seed orchard of Pinus massoniana was established in Guiyang using the 2nd generation seedlings.To better understand their genetic diversity and further utilize them for breeding,87 samples were collected and analyzed using 11 screened ISSR primers.A total of 249 markers from 200 to 2 000 bp were generated and 246 of them(98.80%) were polymorphic markers.Two geographic seed origins were clearly defined and they were Chengbu and Guangxi groups.Within Chengbu group,seeds collected from Hunan and adjacent Guizh... |
| |
| Keywords: | ISSR |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
