拮抗细菌对番茄植株抗灰霉病的诱导

通过拮抗细菌多粘类芽孢杆菌W3、Y2和地衣芽孢杆菌W1 0诱导接种和灰霉病菌挑战接种试验 ,明确了 3菌株培养液和去菌液对番茄植株抗灰霉病的诱导作用。 3株拮抗细菌的诱导抗病效果为 2 3 .4%～ 64.5% ,其中W3诱导作用最强。W 3培养液及其去菌液处理后 5d ,诱导效果达最大值 ,且 1 2d后仍有诱抗作用。在 1 0 2 ～ 1 0 1 0 cfu/ml范围内 ,拮抗细菌的诱导活性随浓度增加而增强 ,以≥ 1 0 8cfu/ml的效果最好。拮抗细菌处理叶上部各叶片间诱抗效果无明显差异。

Induced Resistance to Gray Mould in Tomato Plant by Antagonistic Bacteria

Systemic resistance to gray mould (Botrytis cinerea) in tomato leaves was induced by antagonistic bacteria Paenibacillus polymyxa W3, Y2 and Bacillus licheniformis W10 and their metabolites. Induced resistance effects were from 23.4% to 64.5% as expressed by reduced lesion area. Among 3 antagonistic bacteria, W3 was the best for the induction. After tomato leaves were treated by W3 and its metabolites, induced resistance reached the maximum at the fifth day, and lasted over twelve days. Induced resistance was gradually enhanced along with the increase in bacterial concentration, and reached peak at the concentration of 10~8cfu/ml. There were no significant differences in induced resistance effects among the leaves above the treated leaf.