春石斛组培快繁技术研究

春石斛花朵繁密,花色艳丽,花期长,是东南亚市场上极受欢迎的高档盆花。为了发展春石斛优质种苗的规模化生产,满足国内市场对春石斛的需求,以春石斛的茎段为外植体,以MS为基本培养基,就植物激素对春石斛侧芽诱导、侧芽生长以及继代增殖的影响进行了较全面的研究。结果表明,BA0.5 mg/L+NAA0.1 mg/L的植物激素组合最有利于侧芽的诱导;BA0.5 mg/L+2.4-D0.2 mg/L植物激素组合对侧芽生长最有利;而BA 3.0 mg/L+NAA 0.2 mg/L植物激素组合对侧芽继代增殖促进效果最佳。

Study on the Micropropagation of Dendrobium nobile

The flowers of Dendrobium nobile are dense,the color is gorgeous and the flowering season is long.It is welcomed in the southeast Asia market.In order to develop the scale production of Dendrobium nobile to satisfy the demand of domestic market,we used the stem section as explants and MS medium as primary medium to study the effect of phytohormones on the induction growth and subculture of mixed bud.The results indicated that BA?0.5?mg/L+NAA?0.1?mg/L can improve the induction of the mixed bud,BA?0.5?mg/L+2,4-D?0.2?mg/L can improve the growth of mixed bud,and BA?(3.0)?mg/L+NAA?0.2?mg/L was the best to improve the mixed bud subculture.