| 18个黑麦草品种(系)的RAPD分析 |
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| 引用本文： | 董晓宁,张晓佩,李文杨.18个黑麦草品种(系)的RAPD分析[J].福建农业学报,2009,24(3):266-269. |
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| 作者姓名： | 董晓宁 张晓佩 李文杨 |
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| 作者单位： | 福建省农业科学院畜牧兽医研究所,福州,350013 |
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| 摘 要： | 用RAPD分子标记技术对18个黑麦草(Loluim sp)品种进行遗传多样性研究.从110个10 bp的随机引物中筛选获得26个多态性引物,然后对18份黑麦草种质资源的基因组DNA进行扩增,共获得197条DNA扩增片断带.其中多态性带187条,平均多态检出率为94.9%.18份材料的Jaccard相异系数为0～0.846 7,采用UPGMA法聚类分析,结果显示:雅晴、特高、沃土、潘多拉、安格斯、意大利黑麦草、邦德、普通一年生、嘉宝、绿神、普通二倍体、小黑麦为一群,匹克、凯琳特、顶峰、明星、首相为一群,冬牧70为单独一群.
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| 关 键 词： | 黑麦草 种质资源 遗传多样性 |
Genetic diversity of 18 ryegrass varieties determined by RAPD analysis |
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| DONG Xiao-ning,ZHANG Xiao-pei,LI Wen-yang.Genetic diversity of 18 ryegrass varieties determined by RAPD analysis[J].Fujian Journal of Agricultural Sciences,2009,24(3):266-269. |
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| Authors: | DONG Xiao-ning ZHANG Xiao-pei LI Wen-yang |
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| Institution: | DONG Xiao-ning,ZHANG Xiao-pei,LI Wen-yang(Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China) |
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| Abstract: | RAPD molecular-marker technique was used to detect the genetic diversity among 18 ryegrass germplasms.Their genomic DNA were tested with RAPD using 26 10-mer primers screened from 110 arbitrary 10-mer primers.A total of 197 RAPD bands were obtained,in which 187 were polymorphic.The average percentage of polymorphic bands was 94.9%.The variation range of genetic distances was from 0 to 0.846 7.By a UPGMA cluster analysis on the RAPD molecular-markers,these ryegrass varieties were divided into three groups:(1... |
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| Keywords: | RAPD |
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