| 镰形扇头蜱重组肌钙蛋白I抗蜱免疫保护效果的初步分析 |
| |
| 引用本文： | 周勇志,李庄,石磊,郭凤璕,周金林.镰形扇头蜱重组肌钙蛋白I抗蜱免疫保护效果的初步分析[J].中国兽医寄生虫病,2010,18(1):51-55. |
| |
| 作者姓名： | 周勇志 李庄 石磊 郭凤璕 周金林 |
| |
| 作者单位： | 中国农业科学院上海兽医研究所,农业部动物寄生虫学重点开放实验室,上海,200241 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| |
| 摘 要： | 为了评估镰形扇头蜱肌钙蛋白I（TroponinI，TnI）作为候选疫苗分子的潜力，应用肌钙蛋白I重组蛋白进行了抗蜱免疫实验。将重组蛋白按常规方法免疫新西兰兔，三次免疫后分别用若蜱、成蜱进行攻虫实验，观察其免疫保护效果。结果发现蜱的吸血行为受到一定的影响，与对照相比，若蜱和成蜱吸附率、饱血率、饱血体重和死亡率有显著差异，而在饱血时间上差异不显著，说明TnI基因的重组表达产物具有一定的免疫保护作用。
|
| 关 键 词： | 镰形扇头蜱 肌钙蛋白I 免疫保护 |
ANTI-TICK EFFECT ANALYSIS OF TROPONIN PROTEIN I FROM RHIOICEPHALUS HAEMAPHYSALOIDES |
| |
| ZHOU Yong-zhi,LI Zhuang,SHI Lei,GUO Feng-xun,ZHOU Jin-lin.ANTI-TICK EFFECT ANALYSIS OF TROPONIN PROTEIN I FROM RHIOICEPHALUS HAEMAPHYSALOIDES[J].Chinese Journal of Veterinary Parasitology,2010,18(1):51-55. |
| |
| Authors: | ZHOU Yong-zhi LI Zhuang SHI Lei GUO Feng-xun ZHOU Jin-lin |
| |
| Institution: | ZHOU Yong-zhi,LI Zhuang,SHI Lei,GUO Feng-xun,ZHOU Jin-lin (Key Laboratory of Animal Parasitology of Ministry of Agriculture,Shanghai Veterinary Research Institute,CAAS,Shanghai 200241,China) |
| |
| Abstract: | Recombinant Troponin I(TnI) protein ofRhioicephalus Haemaphysaloides was purified and used to immunize rabbits three times to evaluate its potential as an anti-tick vaccine candidate.Vaccinated rabbits were then exposed to unfed nymph and adult ticks,respectively.The results showed significant difference in the attachment rate,feeding rate and engorged body weight of fed female between vaccinated and control rabbits.There was no significant difference in feeding time between two groups.This preliminary stud... |
| |
| Keywords: | Rhipicephalus haemaphysaloides Troponin I(TnI) vaccine candidate |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
