苹果SBP基因家族生物信息学分析

首先利用生物信息学方法对苹果42条SBP蛋白序列的系统发生和SBP基因组定位进行分析,然后对其氨基酸组成成分、理化性质以及二级和三级结构进行预测和分析,同时还分析了苹果与拟南芥的SBP基因家族之间的联系。结果显示着42条蛋白序列与拟南芥16条SBP蛋白序列一起被分成了7个亚族,拟南芥与苹果SBP基因间具有较高的保守性。基因组定位结果显示42条SBP基因分布在12条染色体上。研究还发现不同亚族间氨基酸数目、氨基酸序列疏水性存在一定的差异;二级结构预测分析发现,42条氨基酸序列以随机卷曲和α-螺旋为主要组成部分,而且42条氨基酸序列三维结构相似。

Bioinformatics Analysis of SBP Gene Family in Apple

This article firstly analyzed the phylogenesis of 42 SBP protein sequences and the localization of SBP genome in apple by using bioinformatics method, and then predicted and analyzed their amino acid composition, physical and chemical characteristics, as well as secondary and tertiary structures, meanwhile analyzed the relation between the SBP gene family in apple and that in Arabidopsis thaliana. The results indicated that the 42 protein sequences in apple and 16 SBP protein sequences in Arabidopsis thaliana could be divided into 7 subtribes, which illuminated that SBP genes had high conservatism between apple and Arabidopsis thaliana. It was also found that these 42 SBP genes distributed on 12 chromosomes. There were some differences in the number of amino acid and hydrophobic quality of amino acid sequences among different subfamilies. The predictive results of secondary structure found that the main compositions of 42 amino acid sequences were randomly curled and α - helix, and the tertiary structure of all 42 amino acid sequences was similar.