马铃薯脱毒快繁抗菌污染的研究

分别加入10、20和50 mg/L的氨苄青霉素、硫酸链霉素、克林霉素及利用3种抗生素(20 mg/L)分别和病毒唑(10 mg/L)的配合使用,研究抗生素及抗生素浓度在马铃薯茎尖剥离及快繁过程中的综合影响。实验结果表明在茎尖剥离阶段,50 mg/L的链霉素对细菌的抗性最好;抗生素的不同浓度对霉菌抗性的效果不同,抗生素种类和浓度的互作对霉菌具有抗性效应,以使用10mg/L的链霉素对霉菌的抗性最好,链霉素 病毒唑组合在茎尖剥离时期对霉菌和细菌的抗性最好;在快繁阶段,以使用20 mg/L克林霉素对霉菌和细菌的抗性最好,但抗生素及抗生素 病毒唑的配比措施对组培苗的生根数、黄化率、生根率均有负效应,所以在马铃薯组培快繁阶段的污染控制主要靠控制环境和操作规程来达到。

Preliminary study on repressing contamination in apical meristem culture and plantlet of potato

The resistance to contamination from both bacteria and fungi had played an important role and become constrainable factors during in apical meristem culture and plantlet propagation of potato.The controversy between to prevent from the contamination by antibiotic and to make a negative impact in the procedure for potato had been argued.To balance the profit between using antibiotics and increasing productivity of potato,two trails,one of is three kind of antibiotics,Amoxycillin(AC),Streptomycin Sulfate(SS) and Clindamycin Hydrochloride(CH),with three different concentration,50,20,10 mg/L,in respectively and other one is the combination both the three antibiotics plus Ribavirin(RB) with 20,10 mg/L,in respectively were employed and mingled into medium during apical meristem culture and plantlet propagation of potato.The results shown that the SS with 50 mg/L resisted to bacteria significantly.For mildew of fungi resistance,the effect is variable significant among three kind of antibiotic and different concentration from different antibiotic.Meanwhile,the interactive effect of resistance to mildew between antibiotic and concentration is significant that the 10 mg/L of SS was best among different combinations.During plantlet propagation,both the three kind of antibiotics and three kind of concentration performed a significant effect of resistance to bacteria and shown that the productivity can be increased by adjustable antibiotics and its concentration.The result also suggested that the combination between antibiotics and RB is not only resist to contamination significant but also affect to other important traits,include number of roots,etiolated plantlet and shoot of roots,with negative effect significant.The approach and strategy both apical meristem culture and plantlet propagation of potato in practice has been discussion.