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妊娠期奶山羊下丘脑GnRH和OT免疫反应双标记细胞的分布
引用本文:陈树林,林磊,蒋田园,雷治海,李文献,曾明华,张艳,范光丽,隋治国.妊娠期奶山羊下丘脑GnRH和OT免疫反应双标记细胞的分布[J].畜牧兽医学报,2007,38(9):966-971.
作者姓名:陈树林  林磊  蒋田园  雷治海  李文献  曾明华  张艳  范光丽  隋治国
作者单位:1. 西北农林科技大学动物科技学院,杨凌,712100
2. 南京农业大学动物医学院,南京,210095
3. 河南省乳品工程技术研究中心,郑州,450008
基金项目:国家自然科学基金(30170683);陕西省自然科学基金(2006C130);河南省乳品工程技术研究中心科研基金(2006001)
摘    要:为了探讨促性腺激素释放激素(GnRH)和催产素(OT)是否在下丘脑细胞中共存,采用免疫组织化学双标记法对妊娠期奶山羊下丘脑中GnRH与OT的分布进行了检测。结果显示:室旁核、视上核、视前交叉上核、弓状核、下丘脑外侧区、乳头体内侧核、乳头体后核等核团(区)有GnRH和OT免疫反应双标记细胞,在妊娠的不同时期,GnRH和OT免疫反应双标记细胞数量有显著差异。这些结果为GnRH与OT相互调节提供了形态学证据。

关 键 词:促性腺激素释放激素  催产素  下丘脑  妊娠奶山羊  免疫组化双标记
文章编号:0366-6964(2007)09-0966-06
修稿时间:2006-10-27

Localization of Double-labeled Immunoreactive Cells of Gonadotropin Releasing Hormone and Oxytocin in Hypothalamus of Dairy Goat during Pregnancy
CHEN Shu-lin,LIN Lei,JIANG Tian-yuan,LEI Zhi-hai,LI Wen-xian,ZENG Ming-hua,ZHANG Yan,FAN Guang-li,SUI Zhi-guo.Localization of Double-labeled Immunoreactive Cells of Gonadotropin Releasing Hormone and Oxytocin in Hypothalamus of Dairy Goat during Pregnancy[J].Acta Veterinaria et Zootechnica Sinica,2007,38(9):966-971.
Authors:CHEN Shu-lin  LIN Lei  JIANG Tian-yuan  LEI Zhi-hai  LI Wen-xian  ZENG Ming-hua  ZHANG Yan  FAN Guang-li  SUI Zhi-guo
Abstract:In the present study,double-labeled immunohistochemistry were adopted to explore co-localization of gonadotropin releasing hormone(GnRH) and oxytocin(OT) in cells of hypothalamus of dairy goat during pregnancy.The results showed that neurons which were double positive with both antigens were localized in nuclei such as paraventricular nucleus,supraoptic nucleus,suprachiasmatic nucleus,arcuate nucleus,lateral hypothalamic area,medial mammillary nucleus and posterior mammillary nucleus.The number of these neurons was significantly changed during different period of gestation.These results provide morphological evidence for a direct modulation between GnRH and OT systems.
Keywords:gonadotropin releasing hormone  oxytocin  hypothalamus  dairy goat during gestation  double-labeled immunohistochemistry
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