桂花品种香气成分的GC/MS指纹图谱研究

 采用固相微萃取(SPME) —气相色谱/质谱(GC/MS) 联用技术分析不同品种桂花的香气成分, 从金桂中鉴定出20种, 银桂中鉴定出13种, 丹桂中鉴定出9种香气成分, 3种桂花均含有顺式罗勒烯、乙酸- 4-己烯酯、顺式芳樟醇氧化物、反式芳樟醇氧化物、β-芳樟醇、α-紫罗酮、β - 紫罗酮和γ-癸酸内酯这8种化合物。将鉴定的金桂、银桂和丹桂作为对照样本, 运用相似度评价法和主成分分析法对各桂花样本指纹图谱进行化学模式识别研究。结果表明, SPME-GC/MS是一种可用于分析不同品种桂花香气成分差异的简单易行的方法, 利用色谱指纹图谱相似度评价法和主成分分析法可以对不同品种的桂花样本进行归类和鉴别。

GC/MS Fingerprint Analysis of Osmanthus fragrans Lour. in Different Varieties

The headspace constituents emitted from different varieties of fresh Osmanthus fragrans Lour. flowers were investigated by means of headspace solid-phase microextraction (HS-SPME) coupled to gas chromatography/mass spectrometry (GC /MS). In this study 20 volatile compoundswere identified in Osmanthus fragrans var. thunbergii, 13 volatile compounds were identified in Osm anthus frag rans, 9 volatile compounds were identified in Osmanthus fragrans f. au rantiacus. (Z)-ocimene, 4-hexenyl acetate, cis-linalol oxide, trans-linalool oxide, β-linalool, α-ionone, β-ionone, γ-decalactone were the same volatiles released from fresh flowers of three different Osmanthus fragrans Lour. The finger prints were analyzed by similarity evaluation and princip le component analysis to conduct the chemical pattern recognition. The results indicated that headspace SPME-GC /MS afforded a simp le samp ling method for identification of the main volatile fragrance from different varieties of fresh Osm anthus fragrans Lour. flowers. The fragrans of Osmanthus fragrans Lour. flowers not only had some resemblance, but also had some difference. The different varieties of fresh Osmanthus fragrans Lour. flowers were distinguished and classified by finger prints similarity evaluation andp rincip le component analysis.