| 玉米淀粉分支酶sbe2a基因反义载体的构建及其转基因初步研究 |
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| 引用本文： | 关淑艳,王丕武,刘广娜,刘慧婧,赵丽娜,郭树成. 玉米淀粉分支酶sbe2a基因反义载体的构建及其转基因初步研究[J]. 吉林农业大学学报, 2009, 31(4) |
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| 作者姓名： | 关淑艳 王丕武 刘广娜 刘慧婧 赵丽娜 郭树成 |
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| 作者单位： | 吉林农业大学生物技术中心,长春,130118;农安县农业技术推广中心,农安,130200 |
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| 基金项目： | 吉林省财政育种项目,吉林农业大学科研启动基金项目 |
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| 摘 要： | 应用PCR技术克隆了玉米淀粉分支酶sbe2a基因片段,并将其克隆到pMD18-T载体,对重组子进行PER检测和限制性内切酶分析,并进行序列比对.结果表明:克隆片段长度为562 bp.将该基因反向插入到pWGLL载体Aetin-pro启动子下游,构建高效反义表达载体,通过花粉管通道法将其导入玉米自交系铁7922,经PCR检测初步证明外源基因已整合到玉米基因组中.
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| 关 键 词： | 玉米 淀粉分支酶基因sbe2a 克隆 反义载体 |
Construction of Antisense Expression Vector on Corn Starch Branching Enzymes Gene sbe2a and Initial Research of Transgenic Corn |
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| GUAN Shu-yan,WANG Pi-wu,LIU Guang-na,LIU Hui-jing,ZHAO Li-na,GUO Shu-cheng. Construction of Antisense Expression Vector on Corn Starch Branching Enzymes Gene sbe2a and Initial Research of Transgenic Corn[J]. Journal of Jilin Agricultural University, 2009, 31(4) |
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| Authors: | GUAN Shu-yan WANG Pi-wu LIU Guang-na LIU Hui-jing ZHAO Li-na GUO Shu-cheng |
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| Abstract: | cDNA segment of corn starch branching enzymes gene sbe2a was obtained by PCR technique and cloned into pMD18-T vector.The recombinant clone was detected by PCR technique and analyzed by the restriction enzyme.A full length of cDNA gene was sequenced.The results showed that the length of the clone sequence was 562 bp.The fragment of anti-sense gene was inserted into Actin-pro promoters of pWGLL to construct over-expression vector.Then it was transformed into corn inbred lines TIE 7922 by using Pollen-tube Pa... |
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| Keywords: | corn starch branching enzymes genes sbe2a cloning anti-sense expression vector |
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