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| 利用AFLP对桃遗传连锁图的加密及加密前后连锁图的比较分析 | |
| 引用本文： | 辛翠花,朱美云,吴俊,张开春,姜立杰,周晓航,张晓明.利用AFLP对桃遗传连锁图的加密及加密前后连锁图的比较分析[J].农业生物技术学报,2006,14(3):387-390. |
| 作者姓名： | 辛翠花 朱美云 吴俊 张开春 姜立杰 周晓航 张晓明 |
| 作者单位： | 1. 北京市农林科学院林业果树研究所,北京,100093;内蒙古农业大学林学院,呼和浩特,010019;中国农业大学农学与生物技术学院,北京,100094 2. 内蒙古农业大学林学院,呼和浩特,010019 3. 北京市农林科学院林业果树研究所,北京,100093 |
| 基金项目： | 国家高技术研究发展计划(863计划);北京市自然科学基金 |
| 摘 要： | 以桃(Pruns persica (L.) Batsch)品种大久保与兴津油桃杂交F2 代109株后代为作图群体，利用AFLP分子标记对已构建的桃遗传连锁图进行加密，在原有作图数据的基础上又增加了35个符合孟德尔分离比例的AFLP标记，其中28个新的标记被加到了遗传连锁图上。应用Mapmaker分析软件将所有的144个标记构建到包含11个连锁群的连锁图谱中，连锁群的总长度为1167.6 cM，覆盖了桃基因组范围的97.3%。连锁群的平均长度为106.15 cM，标记间的平均距离为12.04 cM。与已构建的连锁图比较，发现9个新的标记被加到了第9连锁群，而原有的5个标记丢失了；在第6连锁群，不仅标记间的顺序改变了，而且标记间的平均距离也加大了。另外，第3和第11连锁群间、第1和第10连锁群间，一些标记的位置发生了变化，并对产生这些差异的原因进行了初步分析。 |
| 关 键 词： | 桃 遗传连锁图 AFLP |
| 文章编号： | 1006-1304(2006)0-00-0 |
| 收稿时间： | 2005-6-1 |
| 修稿时间： | 2006-4-26 |
Density-increasing of Peach Genetic Map by AFLP Technique and Comparative Analysis between the Density-increased and the origin Peach Genetic Maps |
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| XIN Cui-hua,ZHU Mei-yun,WU Jun,ZHANG Kai-chun,JIANG Li-jie,ZHOU Xiao-hang,ZHANG Xiao-ming.Density-increasing of Peach Genetic Map by AFLP Technique and Comparative Analysis between the Density-increased and the origin Peach Genetic Maps[J].Journal of Agricultural Biotechnology,2006,14(3):387-390. | |
| Authors: | XIN Cui-hua ZHU Mei-yun WU Jun ZHANG Kai-chun JIANG Li-jie ZHOU Xiao-hang ZHANG Xiao-ming |
| Institution: | 1. Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100093, China;2. College of forestry, Inner Mongolia Agricultural University, Huhhot 010019, China; 3. College o f Agronomy and Biotechnology, China Agricultural University, Beijing 100094,China |
| Abstract: | AFLP markers were used to increase density of the peach (Pruns persica (L.) Batsch) genetic map based on a F2 of 109 progenies derived from the cross of cultivars Okubo and Xingjin. Thirty and five new markers were obtained and 28 markers were added to the genetic map constructed previously. A total of 144 markers were assigned to 11 linkage groups (LOD 3.0), the total length of linkage map was 1167.6 covering 97.3% of the peach genome. The average length of 11 groups and the average interval between markers was 106.15 cM and 12.04 cM respectively. Compared with the previous linkage map, 9 new markers were assigned to group 9, but 5 markers previously located at this group were lost. At group 6, not only the order of the loci was changed, but also the average interval distance of them increased. Furthermore, locations of some markers were changed between group 3 and group 11, between group 1 and group 10. These inconsistencies were also discussed. |
| Keywords: | AFLP |
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