Development of PPO inhibitor-resistant cultures and crops |
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Authors: | Li Xianggan Nicholl David |
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Affiliation: | Syngenta Biotechnology, Inc, PO Box 12257, 3054 Cornwallis Road, Research Triangle Park, North Carolina 27709-2257, USA. Xianggan.Li@syngenta.com |
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Abstract: | Recent progress in the development of protoporphyrinogen oxidase (PPO, Protox) inhibitor-resistant plant cell cultures and crops is reviewed, with emphasis on the molecular and cellular aspects of this topic. PPO herbicide-resistant maize plants have been reported, along with the isolation of plant PPO genes and the isolation of herbicide-resistant mutants. At the same time, PPO inhibitor-resistant rice plants have been developed by expression of the Bacillus subtilis PPO gene via targeting the gene into either chloroplast or cytoplasm. Other attempts to develop PPO herbicide-resistant plants include conventional tissue culture methods, expression of modified co-factors of the protoporphyrin IX binding subunit proteins, over-expression of wild-type plant PPO gene, and engineering of P-450 monooxygenases to degrade the PPO inhibitor. |
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