枸杞多糖对双酚A暴露小鼠生精细胞Caspase-3、Bcl-2和Bax蛋白表达的影响

研究枸杞多糖(LBP)对双酚A(BPA)暴露小鼠睾丸生精细胞中Caspase-3、Bcl-2和Bax凋亡蛋白表达的影响.将50只成年雄性昆明小鼠随机分为A、B、C、D、E共5组,每组10只.除正常对照组(A组)注射等量橄榄油外,其余4组小鼠分别腹腔注射20 mg·kg 1的BPA,连续7d,建立生精损伤模型.同时C、D、E组小鼠分别灌服7d不同剂量的LBP(50、100、200 mg·kg-1),正常对照组(A组)和模型组(B组)小鼠灌服等量生理盐水.制备组织切片观察睾丸组织病理学变化,免疫组化法测定睾丸组织Caspase-3、Bax和Bcl-2凋亡蛋白的表达.结果显示,BPA可极显著增加睾丸生精细胞Caspase-3和Bax的阳性细胞数量(P＜0.01),降低Bcl-2的表达(P＜0.05).补充不同剂量LBP后,Caspase-3的阳性表达均极显著低于模型组(P＜0.01).200 mg·kg-1 LBP组生精细胞Bax的阳性细胞数量极显著低于模型组(P＜0.01)；Bcl-2的表达随LBP剂量的增加而提高,其中200 mg·kg1LBP组阳性表达极显著高于模型组(P＜0.01),Bcl-2/Bax比值也随着LBP剂量的增加而上升.结果表明,枸杞多糖通过调节凋亡相关基因的表达,抑制生精细胞凋亡,从而缓解双酚A引起的雄性生殖损伤.

Effects of LBP on the Expression of Caspase-3, Bcl-2 and Bax Proteins in Spermatogenic Cells of Mice Pretreated with Bisphenol A

The aim of this experiment was to study the effects of LBP on the expression of Caspase-3,Bcl-2 and Bax proteins in spermatogenic cells of mice.Fifty adult male Kunming mice were randomly divided into five groups,which were A,B,C,D and E,with 10 mice in each group.Except the mice in group A which were injected with olive oil as control group,the mice in other four groups were intraperitonealy injected with 20 mg·kg-1 BPA for 7 days.Meanwhile,the mice in groups C,D,E were given different doses of LBP(50,100 and 200 mg·kg-1) by intragastric administration consecutively for one week,the mice in control(A) group and model(B) group were treated with physiological saline.Histopathological changes of the testicular tissue were observed,immunohistochemical technique was applied to detect the expression of Caspase-3,Bcl-2 and Bax proteins.Results showed that BPA can enhance the expression of Caspase-3 and Bax proteins(P<0.01),diminish the expression of Bcl-2(P<0.05).After supplement with different doses of LBP,the expression of Caspase-3 were all significantly decreased in C,D and E groups than that in the model group(P<0.01).With the dose of LBP increasing,the expression of Bax was significantly decreased and the expression of Bcl-2 gradually increased than that in the model group especially in the 200 mg·kg-1 LBP group(P<0.01).Compared with the model group,Bcl-2/Bax ratio also increased obviously in groups B,C and D.Results suggest that LBP can restrain spermatogenic cell apoptosis through regulating the expression of apoptosis-related genes,thus alleviate the reproductive damage induced by BPA on adult male mice.