猪NAP1L5基因克隆、序列鉴定及在不同妊娠时期胎盘中的差异表达分析

旨在获得猪NAP1L5基因的序列特征、组织表达及生物学功能等相关信息.本试验通过RT-PCR克隆了猪NAP1L5基因,并运用生物信息学方法分析了不同物种序列进化关系,运用荧光定量PCR技术检测了NAP1L5在梅山猪不同妊娠时期胎盘中的表达.序列分析结果表明,猪NAP1 L5基因CDS全长579 bp,编码193个氨基酸.其核苷酸序列与牛的相似性为89％,其中氨基酸序列包含一段保守的核小体组装蛋白(Nucleosome assembly protein,NAP)特征序列.荧光定量结果表明NAP1L5基因随着妊娠时期的增长呈上升表达,在26和50 d之间表达量差异极显著(P＜0.01).以上结果支持了NAP1L5基因可能与猪胎儿营养获取及早期发育有关,为进一步研究猪NAP1 L5基因的功能奠定了基础.

Cloning, Sequence Identification and Expression Analysis of NAP1L5 in Porcine Placenta at Different Gestation Stages

The aim of this study was to obtain the information about sequence structure,tissue expression and biology function of porcine NAP1L5 gene.In the present study,the porcine NAP1L5 was cloned by RT-PCR,the sequence was analyzed by bioinformatics method,and its relative mRNA expression was detected by quantitative RT-PCR in porcine placenta at different gestation stages.Sequence analysis showed that the CDS region of the porcine NAP1L5 gene included 579 nucleotides,encoding 193 amino acids,and the sequence similarity of NAP1L5 between the pig and cattle was 89%.The amino acids sequence included the nucleosome assembly protein.The result of quantitative RT-PCR indicated that the expression of porcine NAP1L5 increased with the placenta development and there were significant difference between d26 and d50 of gestation stage(P<0.01).These results suggest that NAP1L5 is probably related to the nutrition absorption and early development of porcine fetus,and make a foundation for the further study on the function of NAP1L5 gene in porcine.