Differential accumulation of high and low molecular weight heat shock proteins in Basmati rice (<Emphasis Type="Italic">Oryza sativa</Emphasis> L.) cultivars |
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Authors: | N Iqbal S Farooq R Arshad A Hameed |
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Institution: | (1) Nuclear Institute for Agriculture and Biology (NIAB), P.O. Box 128, Jhang road, Faisalabad, Pakistan |
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Abstract: | The accumulation of various heat shock proteins (HSPs) and their relationship with the inbuilt cold tolerance observed in
Kashmir Basmati was studied. Five Basmati rice verities (Oryza sativa), Basmati-370, Basmati-Pak, Basmati-198, Basmati-385 and Kashmir Basmati were given temperature shock of 45 and 50°C. Temperature
shocks were given for 16 h in incubator preheated to 45 and 50°C and 85% relative humidity. Proteins were extracted and separated
on 10% acrylamide gels with 1 mm thickness and visualized for protein fractions. Accumulation of 40 kDa HSPs were observed
in all the cultivars, and 20 kDa HSPs specifically in Kashmir Basmati. Small amounts of high molecular weight HSPs were observed
in un-treated (control) plants of Kashmir Basmati, and it increased considerably after heat shock. The 20 kDa HSP was only
expressed in heat-treated Kashmir Basmati. Differences in the expression of heat shock proteins in the tested varieties have
been described in detail. |
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Keywords: | |
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