| 渗透胁迫下水稻根系核仁小分子RNA转录本变化的全基因组表达分析 |
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| 引用本文： | 马廷臣,陈荣军,余蓉蓉,曾汉来,张端品. 渗透胁迫下水稻根系核仁小分子RNA转录本变化的全基因组表达分析[J]. 中国水稻科学, 2012, 26(1): 16-20. DOI: 10.3969/j.issn.1001 7216.2012.01.004 |
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| 作者姓名： | 马廷臣 陈荣军 余蓉蓉 曾汉来 张端品 |
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| 作者单位： | 安徽省农业科学院水稻研究所/安徽省水稻遗传育种重点实验室/国家水稻改良中心合肥分中心;华中农业大学作物遗传改良国家重点实验室;四川农业大学水稻研究所 |
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| 基金项目： | 国家科技支撑计划资助目(2009BADA6B01);转基因生物新品种培育科技重大专项(2011ZX08001-004);国家863计划资助项目(2010AA101801);安徽省农业科学院院长青年创新基金资助项目(11B01070) |
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| 摘 要： | 利用Affymetrix水稻60 k芯片研究了聚乙二醇6000(PEG)模拟干旱时,水稻根系核仁小分子RNA（snoRNA）转录本的表达活性变化。在模拟干旱胁迫下,水稻根系snoRNA转录本表达有很强的品种特异性,干旱敏感品种爱华5号中共有23个snoRNA转录本活性发生变化且均表现为上调表达,而耐旱品种湘丰早119中仅有2个snoRNA转录本表达活性发生变化,也都为上调表达;转录活性发生变化的snoRNA转录本都为C/D框型,且在染色体上存在5处snoRNA簇;爱华5号和湘丰早119在模拟干旱胁迫下表达发生变化的snoRNA转录本有1个转录本重叠,且此转录本在爱华5号中表达活性是湘丰早119中的17.54倍。
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| 关 键 词： | 基因芯片 水稻 根系 核仁小分子RNA 转录本 干旱胁迫 |
| 收稿时间： | 2011-01-09; |
Changes in Small Nucleolar RNA Transcripts in Rice Roots under Osmotic Stress by Microarray |
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| MA Ting-chen,CHEN Rong-jun,YU Rong-rong,ZENG Han-lai,ZHANG Duan-pin. Changes in Small Nucleolar RNA Transcripts in Rice Roots under Osmotic Stress by Microarray[J]. Chinese Journal of Rice Science, 2012, 26(1): 16-20. DOI: 10.3969/j.issn.1001 7216.2012.01.004 |
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| Authors: | MA Ting-chen CHEN Rong-jun YU Rong-rong ZENG Han-lai ZHANG Duan-pin |
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| Affiliation: | 1 Key Laboratory of Rice Genetics and Breeding of Anhui Province/Branch of National Rice Improvement Center/Rice Research Institute,Anhui Academy of Agricultural Sciences,Hefei 230031,China;2 National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,China;3 Rice Research Institute,Sichuan Agricultural University,Chengdu 611134,China;) |
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| Abstract: | The expression level of snoRNA transcripts was investigated under PEG-stimulated drought stress by Affymetrix 60 k rice genome array.The expression of rice snoRNA transcripts was cultivar-specific under drought stress.A total of 23 snoRNA transcripts from Aihua 5 and only two snoRNA transcripts from Xiangfengzao 119 were up-regulated under the osmotic stress.The snoRNA transcripts from two cultivars were box C/D type and presented in five gene clusters on the chromosomes for these snoRNA transcripts.Moreover,an overlap transcript encoding Z274 snoRNA from two cultivars was obtained,but the changed fold of the Z274 snoRNA transcript from Aihua 5 was 16.54 fold more than that of Xiangfengzao 119. |
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| Keywords: | microarray rice roots small nucleolar RNA transcript drought stress |
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