Phaseollidin stored in vacuoles and the phytoalexin response in bean |
| |
Authors: | E Soriano-Richards D Uribe-Salas & G Ibarra-Barrera |
| |
Institution: | Instituto de Investigaciones Quıamp;;acute;mico-Biológicas, Universidad Michoacana SNH, Apartado Postal 50–3, Morelia 58089, México |
| |
Abstract: | In time-course experiments, the amounts of phytoalexin in hypocotyls of the resistant bean cultivar Flor de Mayo were determined after infection with Colletotrichum lindemuthianum . Phaseollin and phaseollidin accumulated as a defence response. However, phaseollidin accumulated earlier than phaseollin and at greater concentrations (155 + 14 compared with 120 + 9 μ g g−1 fresh weight). Phaseollidin was found conjugated as a glucoside in the tissue. Fungal treatment apparently led to a decline of approximately 50% in phaseollidin conjugate after only 7 h after infection. Isolation of vacuoles confirmed the presence of phaseollidin glucosides in this organelle. Treatment of the tissue or isolated protoplasts with a fungal elicitor also produced a decrease, by half, of phaseollidin conjugate concentrations from vacuoles isolated from both sources. The contribution of pre-existing pools of phaseollidin glycoside to the accumulation of this phytoalexin is discussed. |
| |
Keywords: | |
|
|