| 扇贝超氧化物歧化酶的纯化及性质研究 |
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| 引用本文： | 王跃军,孙谧.扇贝超氧化物歧化酶的纯化及性质研究[J].海洋水产研究,1998,19(2):69-75. |
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| 作者姓名： | 王跃军 孙谧 |
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| 作者单位： | 中国水产科学研究院黄海水产研究所!青岛，266071(王跃军，孙谧，向葆卿)，中国科学院上海生物工程研究中心!上海，200233(李民)，青岛海洋大学生命学院!青岛，266003(马英杰) |
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| 基金项目： | 1995～1998山东省科委青岛市科委科学基金 |
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| 摘 要： | 采用加热，盐析及ＤＥＡＥ－ＳｅｐｈａｄｅｘＡ－５０柱层析的方法，从扇贝内脏团中分离得到一种高等电点的超氧化物歧化酶（ＳＯＤ）该酶由２８１个氨基酸组成，等电点为８．３０。纯酶的比活为２６１６．４Ｕ／ｍｇ蛋白，最大紫外吸收峰２７０．５ｎｍ，为Ｃｕ．Ｚｎ－ＳＯＤ，由二个亚基组成，亚基分子量为１５０００Ｄｒ。此外还研究了该酶其他的理化性质。
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| 关 键 词： | 扇贝内脏团 超氧化物歧化酶 纯化 |
PURIFICATION AND CHARACTERIZATION OF SUPEROXIDE DISMUTASE FROM SCALLOP |
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| Wang Yaojun,Sun Mi,Li Min,Ma Yingjie and Xiang Baoqing.PURIFICATION AND CHARACTERIZATION OF SUPEROXIDE DISMUTASE FROM SCALLOP[J].Marine Fisheries Research,1998,19(2):69-75. |
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| Authors: | Wang Yaojun Sun Mi Li Min Ma Yingjie and Xiang Baoqing |
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| Abstract: | Cu.Zn-superoxide dismutase with high isoelectric point was isolated from scallop visceral mass by heat treatment, followed by precipitation with ammonium sulfate and column chromatography. The purfied enzyme consisted of 281 amino acid residues. Determined by the autoxidation of pyrogalloy method, its specific activity was 2 616. 4U/mg. It had two 15 KD homogenous subunits. its pI was 8. 3, and maximal ultraviolet absorption peak was at 270. 5nm.Other characteristics of the purfied enzyme were also analyzed. |
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| Keywords: | Scallop visceral mass Superoxide dismutase Purification |
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