Quantification of Zeaxanthin Dipalmitate and Total Carotenoids in <Emphasis Type="Italic">Lycium</Emphasis> Fruits (Fructus Lycii) |
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Authors: | Yong?Peng Chen?Ma Yawei?Li Kelvin?Sze-Yin?Leung Zhi-Hong?Jiang Email author" target="_blank">Zhongzhen?ZhaoEmail author |
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Institution: | (1) School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong |
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Abstract: | An HPLC-DAD method has been developed to quantitatively analyze for the content of zeaxanthin dipalmitate, a major carotenoid
in Fructus Lycii, in different species of the genus Lycium. Determination was performed using an Alltima C18 column with the mobile phase consisting of acetonitrile and dichloromethane
(42:58). The total contents of carotenoids in these samples were also determined by using UV spectrophotometric assay. Total
carotenoid concentrations of different Fructus Lycii are within the range of 0.03–0.5%. Zeaxanthin dipalmitate is a predominant
carotenoid, comprising 31–56% of the total carotenoids in Fructus Lycii. This study is the first systematic quantification
of the carotenoids in the fruits of different Lycium species. The results demonstrated that these methods are reliable and facile techniques for rapid analysis of carotenoids
for crude drug and plant-derived food supplements. |
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Keywords: | Fructus Lycii HPLC chromatography Lycium Total carotenoids Zeaxanthin dipalmitate |
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