番茄颈腐根腐病抗性鉴定技术的建立及抗性种质资源筛选

[目的]探索番茄颈腐根腐病(Fusarium crown and root rot,FCRR)苗期抗性鉴定技术,并对番茄种质资源及品种进行抗性鉴定分析,挖掘和丰富可利用的抗性资源,为培育抗颈腐根腐病番茄品种打下基础.[方法]以尖镰孢番茄颈腐根腐病专化型病原菌(Fusarium oxysporum f.sp.radici...

Development of Artificial Inoculation Methodology for Evaluation of Resistance to Fusarium Crown and Root Rot and Screening of Resistance Sources in Tomato

【Objective】The objective of this study is to explore the resistance identification technology of Fusarium crown and root rot (FCRR), carry out the resistance identification and analysis of tomato germplasm resources and varieties, enrich the available tomato resources resistant to FCRR, and to lay the foundation for the cultivation of tomato varieties that resistant to FCRR. 【Method】In the present study, four parameters that influence the inoculation effect including inoculum concentration, seedlings stage, environmental temperature and inoculation methods were studied using susceptible line Heinz 1706 infected with Fusarium oxysporum f. sp. radicis-lycopersici (Forl). Subsequently, 100 tomato materials were identified through artificial inoculation at seedling stage as well as the molecular markers linked to the resistance gene. 【Result】The disease incidence and disease index of FCRR increased in a certain range along with the increase of inoculum concentration, and the actual levels of plant resistance could be revealed with the inoculum of 10⁷spores/mL, for which the disease incidence and disease index were 100% and 89.2, respectively. The disease index was not significantly different among different seedling ages when the host was inoculated at 2 to 5 leaf stage. The effects of different environmental temperatures after inoculation on the disease index of FCRR were significantly different and the lower temperature (20℃) condition was more favorable to the occurrence of FCRR. The incidence and disease index were higher and the effect was stable using root dipping and root irrigation methods, which were significantly better than stem injection. The result of inoculation identification suggested that 38 materials showed resistance to FCRR among 100 tomato materials, which could be used for breeding or production in tomato for FCRR resistance. In addition, among the reported markers linked to Frl, SCAR_Frl has the lowest accuracy of 51%, while C2-25 has the accuracy of 59% and the accuracy of PNU-D4 is 83%, which is expected to be used in marker-assisted selection (MAS) for FCRR resistance. 【Conclusion】The established assessment system can identify levels of the resistance to FCRR in tomato seedlings, which can be used for identification and screening of tomato materials.