| 啤酒花矮化类病毒属属级寡核苷酸芯片筛查方法的建立 |
| |
| 引用本文： | 商明清,赵文军,李世访,张永江.啤酒花矮化类病毒属属级寡核苷酸芯片筛查方法的建立[J].植物检疫,2012(2):16-19. |
| |
| 作者姓名： | 商明清 赵文军 李世访 张永江 |
| |
| 作者单位： | 山东省植物保护总站;中国检验检疫科学研究院;中国农业科学院植物保护研究所 |
| |
| 摘 要： | 啤酒花矮化类病毒属是重要的植物类病毒属,目前尚无有效的筛查方法。通过对该属类病毒的核苷酸序列进行分析筛选,设计了8条用于该属类病毒筛查的属级特异性探针并制备了寡核苷酸芯片。应用啤酒花矮化类病毒标准样品对该芯片进行验证,结果表明所建立的属级芯片可以特异性检测啤酒花矮化类病毒,可检测到2ng/μL的总RNA。该芯片可用于啤酒花矮化类病毒属类病毒的筛查,为该属类病毒的检疫与防控提供技术支撑。
|
| 关 键 词： | 啤酒花矮化类病毒属 属级探针 寡核苷酸芯片 |
Establishment of the screening oligonucleotide microarray of Hostuviroid in genus level |
| |
| Shang Mingqing,Zhao Wenjun,Li Shifang,Zhang Yongjiang.Establishment of the screening oligonucleotide microarray of Hostuviroid in genus level[J].Plant Quarantine,2012(2):16-19. |
| |
| Authors: | Shang Mingqing Zhao Wenjun Li Shifang Zhang Yongjiang |
| |
| Institution: | 1.The Plant Protection Union Station of Shandong Province,Jinan 250100,China;2.Chinese Academy of Inspection and Quarantine;3.Institute of Plant Protection,CAAS) |
| |
| Abstract: | hostuviroid is a significant plant viroid genus and has not screening method available at present.For the establishment of the screening oligonucleotide microarray of hostuviroid in genus level,eight genus specific probes from the sequences of hostuviroid viroid were designed.Microarray was constructed based on the probes and identificated with hop stunt viroid standard samples.Results showed a high specificity for detection of hostuviroid viroid and the same detection sensitivity with RT-PCR,which is 2ng/μL of total RNA.The microarray can be used screened and provide technical support of quarantine and control for hostuviroid viroid. |
| |
| Keywords: | Hostuviroid genus level probe oligonucleotide microarray |
| 本文献已被 CNKI 等数据库收录！ |
