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| 卵母细胞扫描电镜和透射电镜样本的制作 | |
| 引用本文： | 武彩红,朱达文,胡新岗,芮荣,戴建军,郑筱峰,张斌. 卵母细胞扫描电镜和透射电镜样本的制作[J]. 安徽农业科学, 2010, 38(1): 182-184 |
| 作者姓名： | 武彩红 朱达文 胡新岗 芮荣 戴建军 郑筱峰 张斌 |
| 作者单位： | 1. 江苏畜牧兽医职业技术学院,江苏泰州,225300;江苏姜曲海种猪场,江苏泰州,225300 2. 江苏畜牧兽医职业技术学院,江苏泰州,225300 3. 南京农业大学动物医学院,江苏南京,210095 4. 上海农业科学院畜牧兽医研究所,上海,201106 |
| 基金项目： | 江苏省自然科学基金(BK2008589);;上海市科技兴农重点攻关项目(沪农科攻字[2003]第14-1号) |
| 摘 要： | [目的]探讨卵母细胞扫描电镜和透射电镜的制作方法，为相关研究提供技术支持。[方法]以猪GV期和体外成熟MII期卵母细胞为研究对象，分别制作扫描电镜样本和透射样本，并观察其超微结构变化。[结果]所用方法需样本量少，电镜下细胞形态结构完好，超微结构清晰。[结论]证实该方法适用于卵母细胞超微结构观察的制样。 |
| 关 键 词： | 卵母细胞 扫描电镜 透射电镜 超微结构 |
Preparing of SEM and TEM Samples for Oocyte |
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| WU Cai-hong et al. Preparing of SEM and TEM Samples for Oocyte[J]. Journal of Anhui Agricultural Sciences, 2010, 38(1): 182-184 | |
| Authors: | WU Cai-hong et al |
| Affiliation: | Jiangsu Animal Husbandry and Veterinary College;Taizhou;Jiangsu 225300 |
| Abstract: | [Objective] The research aimed to explore the manufacturing methods of scanning electron microscope(SEM) and transmission electron microscopy(TEM) for oocyte and provide technical support for related research.[Method] With GV stage and MII stage oocyte as the study object,samples of SEM and TEM were prepared respectively,then changes of the ultrastructure were observed.[Result]The results showed that the method need small number samples,keep intact cell morphology and can see clear ultrastructure.[Conclusio... |
| Keywords: | Oocyte Scanning electron microscope Transmission electron microscope Ultrastructure |
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