石灰、蒌叶处理槟榔对小鼠生殖毒性及体温的影响

为探索添加蒌叶和石灰对槟榔毒副作用的影响,设置蒌叶+槟榔、石灰+槟榔、蒌叶+石灰+槟榔、槟榔4个处理及空白对照(蒸馏水),分别将不同处理的水提液(蒸馏水)对SPF级KM小鼠进行灌胃处理,每天1次,连续灌胃14 d,测定小鼠的生精细胞凋亡指数、Bcl-2与Bax蛋白表达量及体温。结果表明,与空白对照相比,各处理的睾丸曲细精管中生精细胞凋亡指数均增高且差异显著,其中槟榔处理的凋亡指数最大,其次是槟榔+石灰,槟榔+蒌叶+石灰处理最小,槟榔处理的凋亡指数是槟榔+蒌叶+石灰处理的2.6倍;各处理的Bax蛋白表达阳性率均增高,Bcl-2蛋白表达阳性率均降低,其中槟榔处理变化幅度最大,槟榔+蒌叶+石灰槟榔处理最小,槟榔+蒌叶+石灰处理的Bcl-2/Bax值是槟榔处理的1.41倍;各处理的小鼠体温均呈现下降趋势,槟榔+蒌叶+石灰处理体温相对最高且降幅最小,与槟榔处理相反。可见,添加蒌叶或石灰能减弱槟榔对小鼠的毒性作用。

Influence of Lime,Piper betle Processing Betelnut on Mice' Genital Toxicity and Body Temperature

To explore the toxic effects of betelnut after Piper betle and lime were added,the KM mice of SPF were intragastricly administered with water extract solution from Piper betle+betelnut,lime+betelnut, Piper betle+lime+betelnut and betelnut once a day and observed for 14 d. Spermatogenic cell index,Bcl-2,Bax and body temperature of mice were measured. The results showed that spermatogenic cell apoptosis index of treatments were increased and had a significant difference with the control,in which the betelnut treatment was the biggest and was 2 . 6 times of Piper betle + lime + betelnut treatment that was the smallest,and lime + betelnut was between them. The positive expression level of Bax protein of the treatments were up regulated,but Bcl-2 protein was down regulated significantly,which betelnut treatment changed was the biggest,and Piper betle+lime+betelnut was minimum,but the Bcl-2/Bax of the laterwas 1. 41 times of the former. Body temperature of mice in four treatments were lower than CK an showed a trend of decline, Piper betle +lime +betelnut treatment ' s temperature was relatively higher and the smallest decrease,in contrast with betelnut. In short,adding Piper betle or lime to betelnut could weaken genital toxicity in mice.