猪乙脑病毒BHK-21细胞传代株BSF.ZZ-1-p60和BSF.ZZ-3-p60的全基因组序列分析 |
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引用本文: | 滕蔓,胡博,冯丽丽,樊剑鸣,郅玉宝,李秀杰,邓瑞广,罗俊.猪乙脑病毒BHK-21细胞传代株BSF.ZZ-1-p60和BSF.ZZ-3-p60的全基因组序列分析[J].河南农业科学,2016(3):135-140. |
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作者姓名: | 滕蔓 胡博 冯丽丽 樊剑鸣 郅玉宝 李秀杰 邓瑞广 罗俊 |
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作者单位: | 1. 河南省农业科学院 动物免疫学重点实验室/河南省动物免疫学重点实验室/农业部动物免疫学重点实验室,河南 郑州,450002;2. 河南省农业科学院 农业经济与信息研究所,河南 郑州,450002;3. 郑州大学 公共卫生学院,河南 郑州,450001 |
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基金项目: | 国家公益性行业(农业)科研专项(201203082-5),河南省科技攻关计划项目(152102110127),河南省农业技术推广财政补助资金项目(YCN201519111),河南省农科院科研发展专项资金项目(20157817) |
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摘 要: | 为研究猪源乙脑病毒分离株基因遗传稳定性,对猪源乙脑病毒分离株BSF.ZZ-1和BSF.ZZ-3在BHK-21细胞上连续传代60次后的病毒全基因组进行序列测定及分析。结果表明,BSF.ZZ-1-p60、BSF.ZZ-3-p60基因组全长均与各自的亲本毒株BSF.ZZ-1、BSF.ZZ-3一致,均为10 977 nt,在2个病毒基因组的10 701 nt位点处均插入1个碱基G。与亲本毒株相比,2个细胞传代毒株的病毒基因组经连续传代后趋于稳定,均有10个位点发生氨基酸突变,这些突变主要集中在E蛋白区域。核苷酸序列同源性比对分析发现,与其他JEV参考毒株相比,BSF.ZZ-1-p60和BSF.ZZ-3-p60与弱毒疫苗株SA14-14-2的核苷酸序列同源性较高,分别为99.6%和95.9%,其氨基酸序列与猪源野毒分离株SH0601和HW的氨基酸序列同源性较高,分别为98.2%、86.4%和98.3%、86.5%。
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关 键 词: | 猪 乙脑病毒 BHK-21细胞 细胞传代 病毒基因组 序列分析 |
Sequence Analysis of Full-length Genomes of Two Japanese Encephalitis Virus Strains BSF. ZZ-1-p60 and BSF. ZZ-3-p60 Passaged on BHK-21 Cells |
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Abstract: | In this study,the full-length genomes of two Japanese encephalitis virus ( JEV) strains,BSF. ZZ-1-p60 and BSF. ZZ-3-p60 that were isolated from pigs and passaged on BHK-21 Cells,were sequenced and compared with reference strains. The results showed that same to the parental viruses, both of the genomes of BSF. ZZ-1-p60 and BSF. ZZ-3-p60 were 10 977 nt long,with an insert base of G at the 10 701 nt site. Compared to the parental strains, the viral genomes of BSF. ZZ-1-p60 and BSF. ZZ-3-p60 were stabilized after passaged on BHK-21 cells for 60 times,with a total of 10 amino acid mutations which we mainly occurred in E proteins in both strains. Compared to the reference JEV strains,both BSF. ZZ-1-p60 and BSF. ZZ-3-p60 showed highest nucleotide sequence homologies (99. 6% and 95. 9%) to vaccine strain SA14-14-2, while for the amino acid sequences,both strains showed highest homologies (98. 2%, 86. 4% and 98. 3%,86. 5% ) to that of the pig-originated strains SH0601 and HW,respectively. |
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Keywords: | pig JEV BHK-21 cell cell passage viral genome sequence analysis |
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