| 响应面法优化山丹鳞茎总黄酮提取工艺的研究 |
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| 引用本文： | 张昊,邵青,贺晓龙,王延峰.响应面法优化山丹鳞茎总黄酮提取工艺的研究[J].河南农业科学,2016(3):152-156. |
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| 作者姓名： | 张昊 邵青 贺晓龙 王延峰 |
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| 作者单位： | 延安大学 生命科学学院/陕西省区域生物资源保育与利用工程技术研究中心,陕西 延安,716000 |
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| 基金项目： | 陕西省高水平大学建设专项资金资助项目(生物学2012SXTS06)，陕西省科技厅农业攻关项目(2012K01-02)，延安市科技局项目(2012Ks-13) |
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| 摘 要： | 在单因素试验的基础上,采用响应面法优化山丹鳞茎总黄酮提取工艺条件。结果表明,山丹鳞茎总黄酮提取最佳工艺为乙醇体积分数73%、料液比32∶1(m L/g)、回流温度69℃、回流时间120 min。经证明,总黄酮含量试验值(4.685 mg/g)与预测值(4.729 mg/g)基本一致,RSD为0.661%,表明所得工艺参数可靠。
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| 关 键 词： | 山丹 总黄酮 乙醇回流提取 响应面法 |
Optimization of Extraction Process of Total Flavonoids from Lilium pumilum bulb Using Response Surface Methodology |
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| Abstract: | The method of response surface analysis was adopted to optimize the extraction conditions of total flavonoids form Lilium pumilum bulb on the basis of single-factor experiment. The results showed that the optimum technological conditions were ethanol concentration of 73%, material-liquid ratio of 32∶1 , reflux temperature of 69 ℃ and reflux time of 120 min. Under such conditions,the actual value of total flavonoids extracted was 4. 685 mg/g,which was basically coincident with the predicted value 4. 729 mg/g. RSD was 0. 661%,indicating that the optimum process conditions were reliable. |
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| Keywords: | Lilium pumilum total flavonoids reflux extraction with ethanol response surface methodology |
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