长穗偃麦草醇溶蛋白的遗传多样性分析

为进一步发掘长穗偃麦草Thinopyrum elongatum(Host)D.R.Dewey]优异种质,采用酸性聚丙烯酰胺凝胶电泳法(A-PAGE),对分别来自伊朗、土耳其、美国等7个国家的18个长穗偃麦草居群醇溶蛋白的遗传多样性进行了分析。这18个居群中共含有70种醇溶蛋白带型,平均多态性位点百分比(PPB)为75.40%(变幅41.43%~92.86%),平均Nei基因多样性指数(h)为0.241 1(变幅0.127 0~0.332 2),平均Shannon信息指数(SI)为0.361 1(变幅0.195 8~0.497 0)。其中17.35%的遗传变异来自长穗偃麦草居群间,82.65%的遗传变异来自居群内。此外,聚类结果显示,所选材料被分为5组,且聚类结果与材料的地理来源并无密切相关性。以上结果表明,长穗偃麦草的醇溶蛋白位点存在丰富遗传多样性,可用于改良小麦加工品质。

Analysis of Genetic Diversity of Gliadins in Thinopyrum elongatum

In order to exploit the excellent germplasm resource of Thinopyrum elongatum ( Host) D. R. Dewey, the gliadins of 18 populations, collected from Iran, Turkey, America and the other four coun-tries, were analyzed and evaluated using A-PAGE methods. The results indicated that a total of 70 poly-morphic bands were detected among the 18 Thinopyrum elongatum populations, the average of PPB, h and SI were 75. 40% (varied range of 41. 43%—92. 86%), 0. 241 1 (varied range of 0. 127 0—0. 332 2) and 0. 361 1 (varied range of 0. 195 8—0. 497 0), respectively. 17. 35% of molecular variation was among populations, and the great majority of genetic variation (82. 65%) resided within populations. The cluster analysis indicated that all the accessions could be divided into five groups. There was no strong association between the clustering results and the geographical distribution. The above results showed that the gliadins of Thinopyrum elongatum ( Host) D. R. Dewey had rich genetic diversity, and could be utilized to improve wheat processing quality.