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Detection of porcine rotavirus by EM, ELISA and CIET
Authors:J Askaa  B Bloch
Affiliation:Department of Veterinary Virology and Immunology, Royal Veterinary and Agricultural University, Bülowsvej 13, DK-1870 Copenhagen V, Denmark
Abstract:Feces samples from swine-herds with severe problems of neonatal diarrhoea, 3 weeks scours or early weaning diarrhoea were examined for porcine rotavirus using three techniques: EM, ELISA and CIET. Infection with rotavirus was found in about one third of the feces samples, representing more than half of the examined swine-herds. EM and ELISA revealed nearly all the samples found to be positive, while CIET as used here detected only half of them.
Keywords:porcine rotavirus   EM   GIET   ELISA   diarrhoea
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