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虎奶菇菌丝体自溶条件的优化研究
引用本文:黄碧芳,陈婷,黄杰,邱莉莉,林文雄. 虎奶菇菌丝体自溶条件的优化研究[J]. 中国农学通报, 2010, 26(5): 254-256
作者姓名:黄碧芳  陈婷  黄杰  邱莉莉  林文雄
作者单位:福建农林大学生命科学学院,福州,350002
基金项目:基金项目:福建农林大学校青年基金项目“虎奶菇菌丝自溶的差异蛋白组学分析”(06A10).
摘    要:自溶现象广泛存在于各种微生物中,但有关食药用菌虎奶菇(Pleurotus tuber-regium (Fr.) Sing.)菌丝体自溶的研究国内外均未见报道。本文利用液体发酵的方法来培养虎奶菇菌丝体,并分别研究了不同温度、pH值与渗透压、O2供应等因素对其菌丝体自溶的影响。结果表明,在本实验条件下,虎奶菇菌丝体较佳的单因子自溶条件分别为温度40℃、pH 9.0、NaCl浓度3-4%及无氧条件。本实验结果为虎奶菇菌丝体自溶机制的深入研究奠定了基础。

关 键 词:烟草  烟草  控烟  公共政策  价值取向  烟草  
收稿时间:2009-10-14
修稿时间:2009-12-09

Study on the optimization of the autolytic conditions of Pleurotus tuber-regium (Fr.) Sing mycelium
Huang Bifang,Chen Ting,Huang Jie,Qiu Lili,Lin Wenxiong. Study on the optimization of the autolytic conditions of Pleurotus tuber-regium (Fr.) Sing mycelium[J]. Chinese Agricultural Science Bulletin, 2010, 26(5): 254-256
Authors:Huang Bifang  Chen Ting  Huang Jie  Qiu Lili  Lin Wenxiong
Affiliation:(College of Life Science of Fujian Agriculture and Forestry University, Fuzhou 350002)
Abstract:The phenomenon of autolysis is widespread among various kinds of microbes. However, there is hitherto no report on the autolysis of Pleurotus tuber-regium (Ft.) Sing. Liquid fermentation method was applied to culture the Pleurotus tuber-regium (Ft.) Sing mycelia and the effects of temperature, pH, osmotic pressure and oxygen supply on the autolysis of Pleurotus tuber-regium (Ft.) Sing mycelium were examined. The results indicated that the optimal conditions for the mycelium autolysis were 40℃, pH 9.0, 3%-4%NACl and anaerobic fermentation. This study provides the basis on which the further investigation of the Pleurotus tuber-regium (Ft.) Sing mycelium autolysis mechanism can be conducted.
Keywords:Pleurotus tuber-regium (Fr.) Sing  autolytic condition  optimization
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