| matK序列作为DNA条形码在苍耳属中的应用 |
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| 引用本文： | 胡伟毅,邵套喜. matK序列作为DNA条形码在苍耳属中的应用[J]. 杂草科学, 2013, 0(4): 13-16 |
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| 作者姓名： | 胡伟毅 邵套喜 |
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| 作者单位： | [1]连云港出入境检验检疫局,江苏连云港222042 [2]江苏省赣榆县塔山镇农业技术服务中心,江苏连云港222123 |
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| 基金项目： | 江苏出入境检验检疫局科技项目（编号：2012KJ54）. |
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| 摘 要： | 以matK序列作为DNA条形码，对从国外截获的7种苍耳属植物进行物种区分鉴定研究，采用DNeasyOPlantMiniKit试剂盒进行总DNA的提取，应用通用引物对其matK基因进行扩增，测序得到7种苍耳属植物的marK序列，利用MEGA5．1软件对这7种苍耳属植物的matK序列进行比对和分析并构建系统树，结果显示：matK序列能从基因位点层面对苍耳属植物进行区分鉴定。
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| 关 键 词： | matK序列 DNA条形码 苍耳属 |
The appfication of matK sequence as DNA barcode in Xanthium |
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| HU Wei-yi,SHAO Tao-xi. The appfication of matK sequence as DNA barcode in Xanthium[J]. Weed Science, 2013, 0(4): 13-16 |
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| Authors: | HU Wei-yi SHAO Tao-xi |
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| Affiliation: | 2 ( 1. Lianyungang Entry - Exit Inspection and Quarantine Bureau, Lianyungang 222042, China; 2. Ganyu Tashan Agricultural Technology Service Center, Ganyu222042, China) |
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| Abstract: | MatK sequence was used as DNA barcode to distinguish and indentify seven plant species in the genus Xanthi- um of foreign origin. DNeasy Plant Mini Kit was used to extract the total DNA. A universal primer was used to amplify matK genes for sequencing. The matK sequences of the seven Xanthium species were contrasted and analyzed by MEGA 5.1 software, and a phylogenetic tree was constructed. The matK sequences were able to distinguish and indentify Xanthi- um plants at the gene level. |
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| Keywords: | matK sequence DNA barcode Xanthium |
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