超声波对双酶水解玉米蛋白的影响

试验对超声场下双酶水解玉米蛋白的酶解反应条件进行了研究,并与恒温水浴条件下玉米蛋白的水解度进行了对照。单因素试验及正交试验结果表明,中性蛋白酶与木瓜蛋白酶按一定比例复合水解的水解度比单独采用其中的一种有所提高:超声功率为1 000 W,超声时间与间歇时间比为2 s∶30 s,底物浓度3%,总酶浓度(中性蛋白酶与木瓜蛋白酶的酶浓度比为2∶1)5%,pH 7.5的条件下,玉米蛋白的水解度可达58.73%。

Effects of Ultrasound on Double-Enzyme Hydrolysis of Corn Protein

The conditions of double enzyme hydrolyzing corn protein were studied under ultrasonic field. Enzymes are limited in industrial application because they can be inactivated easily. Ultrasound is physical energy and can co accelerate reactions with enzymes in some conditions. The hydrolysis degree was increased  by neutral protease and papain. Degree of hydrolysis in the constant temperature water and the presence of ultrasound were compared. By single factor and orthogonal experiments, the results were as follows: ultrasonic power 1　000 W, time ratio of ultrasound to intermittence 2 s∶30 s, concentration of substrate 3％, enzyme／substrate ratio (the ratio of neutral protease to papain 2∶1)5％, pH 7.5, degree of hydrolysis was 58.73％.