黑龙江部分大豆品种分子ID的构建

以黑龙江13个育种单位6个积温带的83份大豆品种为材料, 选择分布在大豆基因组19个连锁群的43对SSR引物进行检测, 共检测出等位变异157个, 每个引物检测到的等位变异数变化范围为2~7个, 平均为3.65个。将聚丙烯酰胺凝胶电泳得到的谱带统计结果根据等位变异的片段大小数字化, 用自行编制的ID Analysis 1.0软件进行数据分析。结果表明, 仅需9对引物(Satt100、Sat_218、Satt514、Satt551、Satt380、Satt193、Satt191、Satt442、Sat_084)可将83份参试大豆品种完全区分开。构建了一套黑龙江省大豆品种的分子ID。

Establishment of Molecular ID in Soybean Varieties in Heilongjiang

A total of 1 300 cultivars have been bred in China from 1923 to 2005. To well evaluate and use the cultivars, it is necessary to identify scientifically and accurately them by DNA molecular markers due to traditional identification methods can no longer meet requirements. In the paper, 83 cultivars developed by 13 breeding units from six temperature summation zone in Heilongjiang were investigated, 157 polymorphic alleles were detected with 43 SSR primers covering 19 linkage groups of soybean genetic map, for each primer, 2 to 7 allele variations were detected in all cultivars, and 3.65 on average. According to fragment size of allele variation, the data numeralized from the PAGE bands were analyzed by the software ID Analysis 1.0. Nine primers (Satt100, Sat_218, Satt514, Satt551, Satt380, Satt193, Satt191, Satt442, and Sat_084) could be used to identify all 83 soybean cultivars. The PAGE pattern of each cultivar amplified with the 9 primers could be used as a molecular ID of the cultivars. A set of molecular ID were established for 83 soybean cultivars in Heilongjiang.