水稻抗稻瘟病基因Pi-ta和Pi-b多重PCR体系的构建与应用

稻瘟病是我国水稻主产区的重要病害之一, 其主效抗性基因Pi-ta和Pi-b在我国很多稻区表现广谱持久的稻瘟病抗性, 被广泛应用于我国的水稻育种和生产。本研究选用稻瘟病抗性基因Pi-ta和Pi-b及其等位基因的功能标记, 在对22份分别已知抗病基因Pi-ta和Pi-b以及感病基因pi-ta与pi-b组成的水稻品种检测验证基础上, 建立了2套稻瘟病基因多重PCR体系: 体系I同时检测抗病基因Pi-ta与Pi-b, 体系II 同时检测感病基因pi-ta与pi-b, 并利用2套体系对336份高世代育种材料进行检测, 与单标记检测结果比较, 表现稳定可靠, 重复性好。本研究构建的抗稻瘟病基因分子标记多重PCR体系可用于水稻种质资源的快速评价和抗稻瘟病分子标记辅助育种。

Establishment and Application of Multiplex PCR System for Two Blast Resistance Genes Pi-ta and Pi-b in Rice

Rice blast is one of the important diseases in major rice producing areas of our country. The main blast resistance genes Pi-ta and Pi-b showed good resistance spectrum to rice blast in many rice growing areas of China, and has been utilized in rice breeding and commercial production. In this study, two sets of multiple PCR system for the two rice blast genes were established by selecting the functional markers of two resistance genes of Pi-ta and Pi-b and two susceptible genes of pi-ta and pi-b, respectively, based on the verification of the genotype of the two rice blast genes known in 22 rice varieties. System I could detect the two resistance genes of Pi-ta and Pi-b, while system II could detect the two susceptible genes of pi-ta and pi-b. Genotypes of the two rice blast genes in 336 new japonica lines were detected by using the two sets of multiple PCR system. The results were highly consistent with those tested by the conventional single mark detection method, indicating that the two multiplex PCR systems were stable, reliable and time-saving. They may serve as a rapid and efficient method to identify and screen rice germplasm resources and be applied in marker assistant selection for pyramiding multiple genes (QTLs) of rice blast resistance in rice breeding.