| 荧光偏振免疫分析检测粮食及其制品中的真菌毒素研究进展 |
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| 引用本文： | 王战辉,米铁军,沈建忠. 荧光偏振免疫分析检测粮食及其制品中的真菌毒素研究进展[J]. 中国农业科学, 2012, 45(23): 4862-4872. DOI: 10.3864/j.issn.0578-1752.2012.23.013 |
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| 作者姓名： | 王战辉 米铁军 沈建忠 |
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| 作者单位： | 中国农业大学动物医学院/国家兽药基准实验室，北京 100193 |
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| 基金项目： | 国家国际科技合作专项(2009DFA32330);农业部公益性行业科研专项项目(201203040);国家“十二五”科技支撑计划(2011BAK10B01) |
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| 摘 要： | 及时、快速和准确地检测粮食中真菌毒素的污染，从而对其进行严格、科学的控制和处理是保障中国粮食安全和人民身体健康的重要途径。基于特异性分子识别的均相荧光偏振免疫分析技术（Fluorescence Polarization Immunoassay，FPIA）兼具高通量、高速度、高灵敏和易于实现自动化等优点，特别适用于大批量粮食样本的快速筛选。文章综述了近10年来荧光偏振免疫分析检测粮食中真菌毒素的研究进展，并着重讨论了荧光偏振免疫分析的发展方向。
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| 关 键 词： | 荧光偏振免疫分析 真菌毒素 荧光标记物 粮食 |
| 收稿时间： | 2012-03-15 |
Advance in Fluorescence Polarization Immunoassay for the Determination of Mycotoxins in Cereals and Related Products |
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| WANG Zhan-hui,MI Tie-jun,SHEN Jian-zhong. Advance in Fluorescence Polarization Immunoassay for the Determination of Mycotoxins in Cereals and Related Products[J]. Scientia Agricultura Sinica, 2012, 45(23): 4862-4872. DOI: 10.3864/j.issn.0578-1752.2012.23.013 |
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| Authors: | WANG Zhan-hui MI Tie-jun SHEN Jian-zhong |
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| Affiliation: | College of Veterinary Medicine, China Agricultural University/National Reference Laboratories for Veterinary Drug Residue, Beijing 100193 |
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| Abstract: | The timely, fast and accurately detecting of mycotoxins in cereals and, thereby, strictly and scientifically regulation and treatment of these contaminants, are the important ways to guarantee grain supply and protect human health. The fluorescence polarization immunoassay (FPIA) based on specific molecular recognition is in particular suitable to screening of mycotoxins in a large number of cereals samples duo to its characteristics of high-through, high-speed and high-sensitivity. This paper reviews the advance in FPIA for the determination of mycotoxins in cereals in recent 10 years and emphatically discussed the emerging trend of FPIA. |
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| Keywords: | fluorescence polarization immunoassay mycotoxin tracer cereals |
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